LOCALIZED DYNAMIC CHANGES IN CORTICAL BLOOD-FLOW WITH WHISKER STIMULATION CORRESPONDS TO MATCHED VASCULAR AND NEURONAL ARCHITECTURE OF RAT BARRELS
LOCALIZED DYNAMIC CHANGES IN CORTICAL BLOOD-FLOW WITH WHISKER STIMULATION CORRESPONDS TO MATCHED VASCULAR AND NEURONAL ARCHITECTURE OF RAT BARRELS
复制标题
DOI：
10.1038/jcbfm.1993.113
复制
发表时间：
1993-11-01
影响因子：
6.3
通讯作者：
ROVAINEN, CM
中科院分区：
文献类型：
--
作者：
COX, SB;WOOLSEY, TA;ROVAINEN, CM
这一假设，即功能群的神经元在晶须桶连接到一个模块化组织的皮质血管进行了测试。血管内铸型显示皮质毛细血管网络类似于从大脑中动脉供血的须桶图案。在组织学切片中，致密毛细血管明显局限于单个桶，并由一个或几个穿透性小动脉供应。在皮质层IV的桶场是本地化的表面动静脉模式。活体血管在麻醉下通过封闭的颅窗用荧光显微镜和SIT或ICCD相机成像。颈动脉内注射异硫氰酸荧光素-葡聚糖、生理盐水或3 μ m乳胶珠后，测量小动脉直径、动静脉通过时间（AVTT）和珠速度的变化。当C排须在4-5 Hz下敲击1 min时，尸检组织学显示，供应对侧C排桶的小动脉中的血流量增加。AVTT在供应邻近皮质的血管中显著减慢。我们假设，脑血管单位供应个别须桶，并在功能上与他们的精确局灶性调节脑血流。
The hypothesis that functional groups of neurons in whisker barrels are linked to a modular organization of cortical vessels was tested. Endovascular casts demonstrated cortical capillary networks resembling the whisker barrel pattern that were fed from the middle cerebral artery. In histological sections, dense capillaries apparently were confined to single barrels and were supplied by one or a few penetrating arterioles. The barrel field in cortical layer IV was localized in relation to surface arteriovenous patterns. Living vessels were imaged through a closed cranial window under anesthesia with a fluorescence microscope and SIT or ICCD cameras. After intracarotid injections of fluorescein isothiocyanate-dextrans, saline, or 3 mum latex beads, changes in arteriolar diameter, arteriovenous transit times (AVTTs), and bead velocities were measured. When row C whiskers were stroked at 4-5 Hz for 1 min, blood flow increased in arterioles that supplied contralateral row C barrels as demonstrated by postmortem histology. AVTTs slowed significantly in vessels supplying adjacent cortex. We hypothesize that cerebral vascular units supply individual whisker barrels and are functionally linked to them for precise focal regulation of cerebral blood flow.