ON THE DNA CONTENT AND PLOIDY OF TRYPANOSOMES
ON THE DNA CONTENT AND PLOIDY OF TRYPANOSOMES
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DOI：
10.1016/0166-6851(82)90049-4
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发表时间：
1982-01-01
影响因子：
1.5
通讯作者：
JAMES, J
中科院分区：
文献类型：
--
作者：
BORST, P;VANDERPLOEG, M;JAMES, J
用荧光细胞光度法和荧光定量吸光法测定了2种锥虫的核DNA和动基体DNA含量。昆虫束状锥虫的细胞核和动基体DNA含量分别为0.095和0.032 pg/非复制细胞，非洲布氏锥虫的细胞核和动基体DNA含量分别为0.097和0.004 pg/非复制细胞。具有2个动质体和1个细胞核的布氏细胞含0.181 pg/核。T.布鲁氏菌和原环培养物的形态没有显著差异。在DNA-DNA复性实验中，T.先前发现布氏杆菌为0.041 pg/细胞核。T.布鲁氏菌显然是二倍体。在T.在大鼠或培养物中生长的布鲁氏菌。
The nuclear and kinetoplast DNA content of 2 trypanosomatids was determined by quantitative absorption and fluorescence cytophotometry of individual Feulgen-pararosaniline stained cells. For the insect trypanosomatid Crithidia fasciculata nuclear and kinetoplast DNA contents of 0.095 and 0.032 pg per non-replicating cell were found. For the African trypanosome Trypanosoma brucei these values are 0.097 and 0.004 pg. A sub-population of T. brucei cells with 2 kinetoplasts and 1 nucleus was found to contain 0.181 pg/nucleus. The DNA values of bloodstream from T. brucei and the procyclic culture form were not significantly different. In DNA-DNA renaturation experiments the haploid amount of DNA in T. brucei was previously found to be 0.041 pg/nucleus. T. brucei is apparently diploid. No sub-population of haploid cells was observed in T. brucei grown in rats or in culture.