Effects of parenteral infusion with medium-chain triglycerides and safflower oil emulsions on hepatic lipids, plasma amino acids and inflammatory mediators in septic rats
Effects of parenteral infusion with medium-chain triglycerides and safflower oil emulsions on hepatic lipids, plasma amino acids and inflammatory mediators in septic rats
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DOI：
10.1054/clnu.1999.0088
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发表时间：
2000-04-01
影响因子：
6.3
通讯作者：
Chen, WJ
中科院分区：
文献类型：
--
作者：
Yeh, SL;Chao, CY;Chen, WJ
本研究旨在探讨使用中链甘油三酯 (MCT) 与红花油 (SO) 乳液作为脂肪源预输注全肠外营养 (TPN) 对脓毒症大鼠肝脂、血浆氨基酸谱和炎症相关介质的影响。将具有颈内导管的正常大鼠分为两组并接受TPN。 TPN 提供 300 kcal/kg/天的热量，其中 40% 的非蛋白质能量以脂肪形式提供。所有 TPN 溶液均为等氮且营养成分相同，但脂肪乳剂由 SO 或 MCT 和大豆油 (9:1) (MO) 的混合物制成。接受TPN 6天后，每组大鼠进一步分为对照组和脓毒症亚组。通过盲肠结扎和穿刺诱导脓毒症，而对照大鼠接受假手术。将所有大鼠分为四组：MCT对照组（MOC，n = 8）、MCT脓毒症组（MOS，n = 8）、红花油对照组（SOC，n = 8）和红花油脓毒症组（SOS，n = 11）。研究结果表明，MOS组的肝脂水平低于SOS组。 SOS组的血浆亮氨酸和异亮氨酸水平显着低于SOC组，但这两种氨基酸在MOC组和MOS组之间没有观察到差异。无论是否输注 MCT 或红花油，脓毒症组的血浆精氨酸水平均显着低于未脓毒症组。然而，无论是 SO 组还是 MO 组，脓毒症组和非脓毒症组之间的血浆谷氨酰胺和丙氨酸水平没有差异。两个脓毒症组之间腹腔灌洗液中白细胞介素-1p、白细胞介素-6、肿瘤坏死因子-α 和白三烯 B-4 浓度没有差异。这些结果表明，预输注MCT的脓毒症大鼠的分解代谢反应不如那些预输注红花油的明显。与红花油相比，TPN联合MCT给药对减少脓毒症引起的肝脏脂肪沉积具有更好的效果。然而，脓毒症前预输注 MCT 对腹腔灌洗液中炎症相关细胞因子或白三烯没有影响。此外，血浆精氨酸似乎是比谷氨酰胺更敏感的脓毒性指标。 (C) 2000 哈考特出版有限公司
This study was designed to investigate the effects of preinfusion with total parenteral nutrition (TPN) using medium-chain triglycerides (MCT) versus safflower oil (SO) emulsion as fat sources on hepatic lipids, plasma amino acid profiles, and inflammatory-related mediators in septic rats. Normal rats, with internal jugular catheters, were divided into two groups and received TPN. TPN provided 300 kcal/kg/day with 40% of the non-protein energy provided as fat. All TPN solutions were isonitrogenous and identical in nutrient composition except for the fat emulsion, which was made of SO or a mixture of MCT and soybean oil (9:1) (MO). After receiving TPN for 6 days, each group of rats was further divided into control and sepsis subgroups. Sepsis was induced by cecal ligation and puncture, whereas control rats received sham operation. All rats were classified into four groups as follows: MCT control group (MOC, n = 8), MCT sepsis group (MOS, n = 8), safflower oil control group (SOC, n = 8), and safflower oil sepsis group (SOS, n = 11). The results of the study demonstrated that the MOS group had lower hepatic lipids than did the SOS group. Plasma leucine and isoleucine levels were significantly lower in the SOS than in the SOC group, but no differences in these two amino acids were observed between the MOC and MOS groups. Plasma arginine levels were significantly lower in septic groups than in those without sepsis despite whether MCT or safflower oil was infused. Plasma glutamine and alanine levels, however, did not differ between septic and non-septic groups either in the SO or MO groups. No differences in interleukin-lp, interleukin-6, tumor necrosis factor-alpha, and leukotriene B-4 concentrations in peritoneal lavage fluid were observed between the two septic groups. These results suggest that catabolic reaction is septic rats preinfused MCT is not as obvious as those preinfused safflower oil. Compared with safflower oil, TPN with MCT administration has better effects on reducing sepsis-induced liver fat deposition. Preinfusion with MCT before sepsis, however, had no effect on inflammatory-related cytokines or leukotriene in peritoneal lavage fluid. In addition, plasma arginine appears to be a more sensitive indicator than glutamine for septic insult. (C) 2000 Harcourt Publishers Ltd.