Multiple treatment of propolis extract ameliorates carbon tetrachloride induced liver injury in rats
Multiple treatment of propolis extract ameliorates carbon tetrachloride induced liver injury in rats
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DOI：
10.1016/j.fct.2008.04.025
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发表时间：
2008-08-01
影响因子：
4.3
通讯作者：
Shukla, Sangeeta
中科院分区：
文献类型：
--
作者：
Bhadauria, Monika;Nirala, Satendra Kumar;Shukla, Sangeeta
蜂胶是一种树脂型蜂蜡制品，由于其具有多种生物活性，已被用作治疗各种疾病的传统药物。本文对蜂胶乙醇提取物(50-400 mg/kg，P.O.)的保肝作用进行了验证。抗四氯化碳(CCl4.0.5ml/kg，P.O.)致大鼠急性肝损伤。已知的保肝药物水飞蓟素作为阳性对照。给予CCl4改变了各种诊断上重要的生化变量。多味蜂胶可显著抑制血清转氨酶、碱性磷酸酶、乳酸脱氢酶、γ-谷氨酰转肽酶、尿素和尿酸的释放；提高肝微粒体药物代谢酶苯胺羟基酶和氨基比林-N-脱甲基酶的活性；显著抑制脂质过氧化，显著提高肝和肾中谷胱甘肽的含量，并使碳水化合物含量(血糖和组织糖原)、蛋白质含量(血清、微囊体和组织蛋白)和脂类含量(血清和组织甘油三酯、血清胆固醇、总胆固醇和组织中的酯化胆固醇)接近对照组。蜂胶治疗还以剂量依赖的方式逆转了CCl4诱导的肝和肾组织结构的严重改变。将200 mg/kg和400 mg/kg剂量的蜂胶的保肝活性与水飞蓟素进行统计学比较，发现在某些参数上蜂胶比水飞蓟素具有更好的保肝效果，从而得出蜂胶的保肝作用。(C)2008年，爱思唯尔有限公司出版。
Propolis, a resinous wax-like beehive product has been used as a traditional remedy for various diseases due to a variety of biological activities of this folk medicine. In the present investigation, an attempt has been made to validate hepatoprotective activity of ethanolic extract of propolis (50-400 mg/kg, p.o.) against carbon tetrachloride (CCl4. 0.5 ml/kg, p.o.) induced acute liver injury in rats. Silymarin, a known hepatoprotective drug was used as a positive control. Administration Of CCl4 altered various diagnostically important biochemical variables. Multiple treatment of propolis significantly prevented the release of transaminases, alkaline phosphatase, lactate dehydrogenase, gamma-glutamyl transpeptidase, urea and uric acid in serum; improved the activity of hepatic microsomal drug metabolizing enzymes, i.e., aniline hydroxylase and amidopyrine-N-demethylase; significantly inhibited lipid peroxidation and markedly enhanced glutathione in liver and kidney as well as brought altered carbohydrate contents (blood sugar and tissue glycogen), protein contents (serum, microsomal and tissue protein) and lipid contents (serum and tissue triglycerides, serum cholesterol, total and esterified cholesterol in tissue) towards control. Propolis treatment also reversed CCl4 induced severe alterations in histoarchitecture of liver and kidney in a dose dependent manner. Hepatoprotective activity of propolis at doses of 200 and 400 mg/kg was statistically compared to silymarin and found that propolis exhibited better effectiveness than silymarin in certain parameters, concluded its hepatoprotective potential. (C) 2008 Published by Elsevier Ltd.