The use of green fluorescent protein-tagged recombinant viruses to test Lettuce mosaic virus resistance in lettuce
The use of green fluorescent protein-tagged recombinant viruses to test Lettuce mosaic virus resistance in lettuce
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DOI：
10.1094/phyto.2002.92.2.169
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发表时间：
2002-02-01
期刊：
影响因子：
3.2
通讯作者：
Redondo, E
中科院分区：
文献类型：
--
作者：
Candresse, T;Le Gall, O;Redondo, E
种子鉴定和使用含有两个可能等位的隐性基因mol（1）和mol（2）之一的品种是控制莴苣花叶病毒（LMV）的主要方法。尽管对于少数LMV分离物，mol（2）赋予大多数分离物抗性，但基因moll或mol（2）赋予耐受性，并且在携带mol的植物中容易检测到病毒积累。这种表型使抗性状态的评估复杂化，特别是对于moll，对于其没有表达真正抗性的病毒株。构建了两种绿色荧光蛋白（GFP）标记的病毒，分别来自非耐药性破坏分离株（LNV-0）和耐药性破坏分离株（LW-E）。用这些重组病毒对101个已知状态的品种进行了评价。使用LMV-0衍生的重组体，携带摩尔的品种的鉴定是简单的，因为与其野生型亲本相反，LMV-0-GFP的系统运动在抗性植物中被废除。该检测试剂盒检测到4例耐药状态错误鉴定的病例。在所有这些情况下，进一步的测试证实先前的抗性状态信息是不正确的，因此在LMV-0-GFP行为和摩尔抗性状态之间观察到100%的相关性。类似地，LMV-E衍生的重组体允许鉴定mol（2）莴苣系，因为其系统运动在mol（2）系中受到限制，但在敏感系或mol 1系中不受限制。标记的病毒能够系统地入侵另一个主机，豌豆，不管它的抵抗状态对马铃薯Y病毒属的另一个成员，豌豆种子传播花叶病毒。因此，这些重组病毒的使用可以大大促进LW抗性评估和加速莴苣育种计划。
Seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mol(1) and mol(2), are the principal control methods for Lettuce mosaic virus (LMV) in lettuce. Although for a few LMV isolates, mol(2) confers resistance with most isolates, the genes moll or mol(2) confer a tolerance, and virus accumulation is readily detected in mol-carrying plants. This phenotype complicates evaluation of the resistance status, in particular for moll, for which there are no viral strains against which a true resistance is expressed. Two green fluorescent protein (GFP)-tagged viruses were constructed, derived from a non-resistance breaking isolate (LNV-0) and from a resistance-breaking isolate (LW-E). An evaluation of 101 cultivars of known status was carried out with these recombinant viruses. Using the LMV-0-derived recombinant, identification of mol-carrying cultivars was simple because, contrary to its wild-type parent, systemic movement of LMV-0-GFP was abolished in resistant plants. This assay detected four cases of misidentification of resistance status. In all these cases, further tests confirmed that the prior resistance status information was incorrect, so that a 100% correlation was observed between LMV-0-GFP behavior and the mol resistance status. Similarly, the LMV-E-derived recombinant allowed the identification of mol(2) lettuce lines because its systemic movement was restricted in mol(2) lines but not in susceptible or in moll lines. The tagged viruses were able to systemically invade another host, pea, irrespective of its resistance status against another member of the genus Potyvirus, Pea seed-borne mosaic virus. The use of these recombinant viruses could therefore greatly facilitate LW resistance evaluation and speed up lettuce breeding programs.