24-hour pattern of circulating prolactin and growth hormone levels and submaxillary lymph node immune responses in growing male rats subjected to social isolation
24-hour pattern of circulating prolactin and growth hormone levels and submaxillary lymph node immune responses in growing male rats subjected to social isolation
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DOI：
10.1385/endo:25:1:41
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发表时间：
2004-10-01
期刊：
影响因子：
3.7
通讯作者：
Cardinali, DP
中科院分区：
文献类型：
--
作者：
Esquifino, AI;Alvarez, MP;Cardinali, DP
为了评估生长大鼠的社会隔离对循环催乳素和生长激素 (GH) 水平的 24 小时节律性以及颌下淋巴结免疫反应的影响，将雄性 Wistar 大鼠从生后第 35 天开始单独笼养或分组（每笼 4-5 只动物）30 天。在 24 小时内以六个时间间隔测定血浆催乳素和 GH 水平、颌下淋巴结淋巴细胞亚群、干扰素 (IFN)-γ 释放以及对刀豆球蛋白 A (Con A) 和脂多糖 (LPS) 的有丝分裂反应。社会隔离导致血浆催乳素和 GH 水平以及淋巴结免疫反应的平均值和 24 小时模式发生变化。分离后，催乳素和GH平均值下降，淋巴结T、B、非T-non B、CD8(+)和CD4(+)/CD8(+)细胞增多，而淋巴结CD4(+)/CD8(+)比率、IFN-γ释放和促有丝分裂反应下降。社会隔离导致每个测试免疫参数的 24 小时节律性被破坏。 CD4+/CD8+ 比值、IFN-γ 释放以及刀豆球蛋白 A (Con A) 和脂多糖 (LPS) 反应与血浆催乳素或 GH 水平显着相关，而 T/B 比值仅与血浆催乳素水平相关。 B、非T-non B和CD4(+)-CD8(+)细胞与血浆催乳素呈负相关。血浆催乳素和 GH 水平的平均值和 24 小时节律性的变化可能与社会隔离对免疫反应的影响有关。
To assess the effect of social isolation of growing rats on 24-h rhythmicity of circulating prolactin and growth hormone (GH) levels and submaxillary lymph node immune responses, male Wistar rats were either individually caged or kept in groups (4-5 animals per cage) for 30 d starting on d 35 of life. Plasma prolactin and GH levels, and submaxillary lymph node lymphocyte subset populations, interferon (IFN)-gamma release and mitogenic responses to concanavalin A (Con A) and lipopolysaccharide (LPS) were determined at six time intervals during the 24 h span. Social isolation brought about changes in mean values and 24-h pattern of plasma prolactin and GH levels and lymph node immune responses. After isolation, prolactin and GH mean values decreased, and lymph node T, B, non T-non B, CD8(+), and CD4(+)/CD8(+) cells augmented, whereas lymph node CD4(+)/CD8(+) ratio, IFN-gamma release and mitogenic responses decreased. Social isolation resulted in disruption of 24 h rhythmicity of every immune parameter tested. CD4+/CD8+ ratio, IFN-gamma release and Concanavalin A (Con A) and lipopolysaccharide (LPS) responses correlated significantly with plasma prolactin or GH levels while T/B ratio correlated with plasma prolactin levels only. B, non T-non B, and CD4(+)-CD8(+) cells correlated negatively with plasma prolactin. Modifications in mean value and 24-h rhythmicity of plasma prolactin and GH levels are presumably involved in the effect of social isolation on immune responsiveness.