Description of Perkinsus beihaiensis n. sp., a new Perkinsus sp. Parasite in Oysters of Southern China
Description of Perkinsus beihaiensis n. sp., a new Perkinsus sp. Parasite in Oysters of Southern China
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Perkinsus beihaiensis n. 的描述
DOI：
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发表时间：
2008
影响因子：
2.2
通讯作者：
K. Reece
中科院分区：
文献类型：
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作者：
Jessica A. Moss;Jie;C. Dungan;K. Reece
摘要。1999-2006年在中国沿海采集牡蛎，采用分子、培养和组织学技术对其寄生虫进行分析。以核糖体RNA基因复合体的内部转录间隔区（ITS）为靶点的聚合酶链反应为基础的检测方法检测了Perkinsus物种的存在。扩增的波金丝虫dna序列分析和系统发育分析表明，一种新的波金丝虫感染了来自福建至广西的香港长尾绦虫、ariak长尾绦虫和其他双壳类宿主。在受影响的牡蛎种群中，这种波金苏氏菌的流行率高达60%。rRNA ITS区、大亚基rRNA和肌动蛋白基因的核苷酸序列分析一致地证实了该珀金苏氏属的隶属关系，但将其与目前公认的珀金苏氏种区分开来。通过组织学观察到2-8 μm直径的印环滋养体等寄生虫细胞类型，并应用Perkinsus属和Perkinsus种特异性原位杂交探针在感染牡蛎组织的组织学切片上一致地标记出相同的Perkinsus sp.细胞。系统发育和组织学的综合结果支持了一种新的寄生虫——北海柏金斯（Perkinsus beihaiensis n. sp）。
ABSTRACT. Oysters were collected from coastal locations in China from 1999–2006 for parasite analyses by molecular, culture, and histological techniques. Polymerase chain reaction‐based assays targeting the internal transcribed spacer (ITS) region of the ribosomal RNA gene complex were performed to detect the presence of Perkinsus species. Sequencing and phylogenetic analysis of amplified Perkinsus sp. DNAs indicated that a novel Perkinsus sp. infects Crassostrea hongkongensis, Crassostrea ariakensis, and other bivalve hosts from Fujian to Guangxi provinces in southern China. Prevalence of this Perkinsus sp. reaches as high as 60% in affected oyster populations. Analyses of nucleotide sequences of the rRNA ITS region and of large subunit rRNA and actin genes, consistently confirmed the genus affiliation of this Perkinsus sp., but distinguished it from currently accepted Perkinsus species. Parasite cell types, such as signet ring trophozoites of 2–8 μm diameter, were observed by histology, and application of both genus Perkinsus and Perkinsus species‐specific in situ hybridization probes consistently labelled the same Perkinsus sp. cells in histological sections from infected oyster tissues. Combined phylogenetic and histological results support the identity of a new parasite species, Perkinsus beihaiensis n. sp.