Are cytokines linked to collagen breakdown during periodontal disease progression?
Are cytokines linked to collagen breakdown during periodontal disease progression?
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DOI：
10.1902/jop.2003.74.2.196
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发表时间：
2003-02-01
影响因子：
4.3
通讯作者：
Gogly, B
中科院分区：
文献类型：
--
作者：
Ejeil, AL;Gaultier, F;Gogly, B
背景：在炎症过程中，由驻留细胞和炎症细胞产生的细胞因子的作用的证据已经很好地建立。本研究的目的是量化健康和患病的人牙龈中胶原纤维占据的面积分数（AA%）和细胞因子如白细胞介素（IL）-1 β、IL-4、IL-6、肿瘤坏死因子（TNF）-α、转化生长因子（TGF）-β和表皮生长因子（EGF）的量，以研究这些细胞因子、胶原降解、和牙龈指数。将来自6名健康对照（第1组）、6名轻度牙龈炎症患者（第2组）、6名中度牙龈炎症患者（第3组）和6名重度牙龈炎症患者（第4组）的牙龈组织标本培养72小时，并使用酶联免疫吸附测定（ELISA）定量培养基中存在的细胞因子。采用Sirius red F3 Ba对24例正常人牙龈石蜡切片进行染色，观察牙龈胶原纤维的分布情况，并通过自动图像分析仪测定牙龈胶原纤维所占面积百分比（AA%）。
Background: Evidence of the role of cytokines produced by resident and inflammatory cells during inflammation is well established. The aim of this study was to quantify in healthy and diseased human gingiva the area fraction (AA%) occupied by collagen fibers and the amount of cytokines such as interleukin (IL)- 1beta, IL-4, IL-6, tumor necrosis factor (TNF) -alpha, transforming growth factor (TGF)-beta, and epidermal growth factor (EGF) to investigate a possible correlation between such cytokines, collagen degradation, and the gingival index.Methods: Gingival tissue specimens from 6 healthy controls (group 1), 6 patients with mild gingival inflammation (group 2), 6 patients with moderate gingival inflammation (group 3), and 6 patients with severe gingival inflammation (group 4) were cultured for 72 hours, and the cytokines present in the culture media were quantified using an enzyme-linked immunosorbent assay (ELISA). Paraffin gingival sections from the 24 subjects were stained with Sirius red F3Ba for visualization of collagen fibers, then the area fraction (AA%) occupied by the gingival fibers was determined by automated image analysis.Results: The present study revealed significant differences (P