IMMUNE BLOT ANALYSIS OF VIRAL SURFACE-PROTEINS IN SERUM AND LIVER OF PATIENTS WITH CHRONIC HEPATITIS-B VIRUS-INFECTION
IMMUNE BLOT ANALYSIS OF VIRAL SURFACE-PROTEINS IN SERUM AND LIVER OF PATIENTS WITH CHRONIC HEPATITIS-B VIRUS-INFECTION
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DOI：
10.1002/jmv.1890290408
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发表时间：
1989-12-01
影响因子：
12.7
通讯作者：
ZUMBUSCHENFELDE, KHM
中科院分区：
文献类型：
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作者：
GERKEN, G;MANNS, M;ZUMBUSCHENFELDE, KHM
肝炎病毒的小表面蛋白和中表面蛋白形成病毒体或22 nm颗粒，两者均被分泌。大表面蛋白本身在人工转染的细胞培养物中保持与细胞结合，除非它伴随有过量的较小蛋白。其体内行为尚未得到充分研究。使用特异性单克隆抗体进行免疫印迹，我们发现慢性病毒携带者的血清中存在丰富的小表面蛋白，并且无论病毒血症如何，肝脏中也存在适量的小表面蛋白。大表面蛋白存在于病毒血症携带者的血清和肝脏中。在非病毒血症携带者中，血清中不存在大蛋白，但在肝脏中，很容易检测到较短形式的大蛋白。这些发现表明病毒表面蛋白的复杂调节机制取决于其他病毒基因产物的表达。
The small and the middle surface proteins of hepatitis virus form either the virion or the 22 nm particle both of which are secreted. The large surface protein by itself remains cell bound in artificially transfected cell culture unless it is accompanied by an excess of the smaller proteins. Its behavior in vivo is not yet well studied. Using specific monoclonal antibodies for immunoblotting, we found an abundance of small surface protein in the serum of chronic virus carriers and moderate amounts in the liver irrespective of viremia. The large surface protein was present in the serum and the liver of viremic carriers. In nonviremic carriers, the large protein was absent from serum, but in the liver a shorter form of the large protein was readily detectable. These findings suggest a complex regulatory mechanism of the viral surface protein depending on the expression of other viral gene products.