CD5+ B cells with the features of subepithelial B cells found in human tonsils
CD5+ B cells with the features of subepithelial B cells found in human tonsils
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DOI：
10.1002/eji.200636887
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发表时间：
2007-08-01
影响因子：
5.4
通讯作者：
Ferrarini, Manlio
中科院分区：
文献类型：
--
作者：
Dono, Mariella;Burgio, Vito Lelio;Ferrarini, Manlio
本研究描述了一种不同于人扁桃体中大多数CD 5（+）B细胞的CD 5（+）B细胞。该细胞分离自体内活化的B细胞，表达活化标志物，具有CD 23（-）、IgM（高）、IgD（低）表面表型，在体外对T细胞非依赖性2型抗原有反应，并在上皮下（SE）区域（相当于脾边缘区（MZ）的扁桃体）中检测到。大多数细胞利用未突变的IG VH基因，尽管也发现了具有突变基因的细胞，这一发现得到了单细胞研究的证实。突变序列在富含CD 27（+）细胞的悬浮液中更常见。在来自相同细胞悬浮液的不同分子克隆中观察到重复的VDJ基因序列，表明原位扩增。这些CD 5（+）B细胞似乎与先前表征的扁桃体CD 5（-）SE B细胞具有共同特征，并且与大多数扁桃体CD 5 + B细胞不同，后者具有滤泡套B细胞的表面表型，缺乏活化标志物，不响应T细胞非依赖性抗原，并且利用未突变的VH基因。这些数据进行了讨论考虑。对B细胞亚群的起源以及MZ与B1细胞的关系提出了看法。
This study describes a CD5(+) B cell that differs from the majority of the CD5(+) B cells from human tonsils. This cell, isolated from in vivo activated B cells, expressed activation markers and featured a CD23(-), IgM(high), IgD(low) surface phenotype, responded to T cell-independent type-2 antigens in vitro, and was detected in the subepithelial (SE) areas, the tonsil equivalent of the splenic marginal zone (MZ). Most of the cells utilized unmutated Ig VH genes, although cells with mutated genes also were found, a finding confirmed by single-cell studies. Mutated sequences were more frequent in suspensions enriched for CD27(+) cells. Repeated VDJ gene sequences were observed in different molecular clones from the same cell suspension, suggesting in situ expansion. These CD5(+) B cells seem to share features with previously characterized tonsil CD5(-) SE B cells and differ from the majority of tonsil CD5+ B cells, which have the surface phenotype of follicular mantle B cells, lack activation markers, do not respond to T cell-independent antigens, and utilize unmutated VH genes. These data are discussed considering the. present views on the origin of B cell subset populations and the relationships between MZ and B1 cells.