Zinc Regulates Lipid Metabolism and MMPs Expression in Lipid Disturbance Rabbits
Zinc Regulates Lipid Metabolism and MMPs Expression in Lipid Disturbance Rabbits
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锌调节脂质紊乱兔的脂质代谢和基质金属蛋白酶的表达
DOI：
10.1007/s12011-015-0367-7
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发表时间：
2015-12-01
影响因子：
3.9
通讯作者：
Gao, Xiuren
中科院分区：
文献类型：
--
作者：
Xu, Chenggui;Huang, Zhibin;Gao, Xiuren
高脂饮食引起的脂质代谢紊乱是一个世界性的问题，在体内可引起炎症和氧化应激。锌被认为是一种抗氧化剂，抗炎剂。由于基质金属蛋白酶2（MMP 2）和基质金属蛋白酶9（MMP 9）的表达在多种病理状态下发生变化，因此我们想了解锌对脂代谢紊乱家兔脂代谢及MMP 2、MMP 9表达的影响。24只雄性新西兰白色家兔随机分为4组。每组6只，分别给予普通饲料、高脂饲料、高脂饲料+锌、普通饲料+锌4种饲料喂养12周。高脂饮食可引起血脂紊乱，使高脂饮食组大鼠血清谷草转氨酶（p< 0.01）和谷丙转氨酶（p< 0.05）升高，补锌可逆转这一现象（p< 0.05）。锌不能降低总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）（p> 0.05），但能降低甘油三酯（TG）和升高高密度脂蛋白胆固醇（HDL-C）（p< 0.01）。高敏C-反应蛋白（hs-CRP）和白细胞介素-6（IL-6）的表达也明显降低（p< 0.01和p< 0.05）。锌减少心外膜脂肪组织，减轻肝脏脂肪变性。锌可抑制体内MMP 2和MMP 9的表达，但不能减轻脂代谢紊乱家兔主动脉脂肪条的严重程度。锌对血脂紊乱家兔肝脏有保护作用，可降低TG、hs-CRP、IL-6，升高HDL-C。锌抑制MMP 2和MMP 9的表达，但不能减轻高脂饮食诱导的主动脉脂肪条的严重程度。
Lipid disturbance induced by high-fat diet is a worldwide problem, and it can induce inflammation and oxidative stress in vivo. Zinc is considered as an antioxidant, anti-inflammatory agent. Since matrix metalloprotease 2 (MMP2) and matrix metalloprotease 9 (MMP9)’s expressions are changed under many pathological conditions, we would like to know how zinc affects lipid metabolism and MMP2, MMP9’s expressions in the lipid disturbance rabbits. Twenty-four male New Zealand white rabbits were randomly divided into four groups. Each group had six rabbits, and they were fed with regular diet, high-fat diet, high-fat diet + zinc, and regular diet + zinc separately for 12 weeks. High-fat diet induced lipid disturbance significantly which raised the level of aspartate aminotransferase (p< 0.01) and alanine transaminase (p< 0.05) in the high-fat diet group, but zinc supplement reversed this phenomenon (p< 0.05). Zinc did not reduce total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) (p> 0.05), but it lowered triglyceride (TG) and raised high-density lipoprotein cholesterol (HDL-C) (p< 0.01). Zinc also reduced high-sensitivity C-reactive protein (hs-CRP) (p< 0.01) and interleukin-6 (IL-6)’s expressions (p< 0.05). Zinc reduced the epicardial adipose tissue and alleviated the hepatic steatosis. Zinc suppressed MMP2 and MMP9’s expressions in vivo, but it did not alleviate the aorta fatty streak’s severity in the lipid disturbance rabbits. Zinc protected the liver, reduced TG, hs-CRP, and IL-6 and raised HDL-C in the lipid disturbance rabbits. Zinc suppressed MMP2 and MMP9’s expressions in vivo, but it did not alleviate the severity of aorta fatty streak induced by the high-fat diet.