The root transcriptome analyses of peanut wild species Arachis correntina (Burkart) Krapov. & WC Gregory and cultivated variety Xiaobaisha in response to benzoic acid and p-cumaric acid stress
The root transcriptome analyses of peanut wild species Arachis correntina (Burkart) Krapov. & WC Gregory and cultivated variety Xiaobaisha in response to benzoic acid and p-cumaric acid stress
复制标题
花生野生种Arachis correntina (Burkart) Krapov 的根转录组分析。
DOI：
10.1007/s10722-019-00859-6
复制
发表时间：
2019-11-13
影响因子：
2
通讯作者：
Du, Lin
中科院分区：
文献类型：
--
作者：
Li, Zhong;Xiong, Faqian;Du, Lin
化感作用是造成连作障碍的主要原因。花生野生种花生Arachim correntina(Burkart)Krapov.&W.C.Gregory比栽培花生对连作障碍的抗性更强，但其对化感物质的反应分子机制尚不清楚。苯甲酸(BA)和对胡萝卜酸(PCA)是已知的化感物质。为了更深入地了解细胞对BA和PCA的响应，我们应用高通量遗传测序技术研究了花生栽培品种小白沙和野生种A.correntina在2 mM BA和PCA存在下的转录组变化。结果表明，沙打旺对BA和PCA胁迫的抗性比小白沙更弱。BA和PCA显著降低了沙打旺的枝条长度和干物重。在BA和PCA胁迫下，与黄酮类化合物生物合成、苯丙素生物合成、植物-病原菌相互作用和植物激素信号转导相关的差异表达基因(DGE)在沙打旺中的转录水平低于小白沙，其中包括苯丙烷和类黄酮类生物合成、解毒酶、生长素反应蛋白、WRKY家族和EF-Hand等基因表达上调较少的基因，这可能是导致沙打旺对BA和PCA胁迫抗性较弱的原因之一。结果表明，对单一化感物质的抗性可能不是沙打旺对连作障碍的抗性原因。此外，在BA和PCA胁迫下，紫云英和小白沙的DGE均在与异黄酮类生物合成和谷胱甘肽代谢相关的途径中显著丰富。这是首次报道了花生栽培品种和野生种在BA和PCA胁迫下DGE的鉴定。这一结果将有助于深入了解花生对化感物质胁迫的响应机制。
Allelopathy is the main cause of continuous cropping obstacles. Peanut wild species Arachis correntina (Burkart) Krapov. & W.C. Gregory is more resistant to continuous cropping obstacle than cultivated peanut, but its molecular mechanism in response to allelochemicals remains unknown. Benzoic acid (BA) and p-cumaric acid (PCA) are known allelochemical. To gain more insight into cellular response to BA and PCA, we applied high-throughput genetic sequencing to study the transcriptome changes of peanut cultivated variety Xiaobaisha and wild species A. correntina in the presence of 2 mM BA and PCA. The result showed that A. correntina resistance to BA and PCA stress was more weaker than Xiaobaisha. BA and PCA decreased significant shoot length and dry matter weight of A. correntina. Differentially expressed genes (DGEs) related to flavonoid biosynthesis, phenylpropanoid biosynthesis, plant-pathogen interaction and plant hormone signal transduction were transcribed less in A. correntina than Xiaobaisha under BA and PCA stress, including the less up-regulated genes involving in phenylpropane and flavonoid biosynthesis, detoxifying enzymes, Auxin responsive protein, WRKY family and Ef-hand, which might contribute to A. correntina's weak resistance to BA and PCA stress. The results showed that the resistance to single allelopathic substance might not be the reason for A. correntina resistance to continuous cropping obstacle. In addition, DGEs of both A. correntina and Xiaobaisha were significantly enriched in the pathways associated with isoflavonoid biosynthesis and glutathione metabolism under BA and PCA stress. This was the first report on identification of DGEs under BA and PCA stress between peanut cultivated variety and wild species. The result would be great helpful to insight into the mechanisms of peanut response to allelochemicals stress.