Effects of ethanol on embryonic and neonatal rat testes in organ cultures.
Effects of ethanol on embryonic and neonatal rat testes in organ cultures.
复制标题
乙醇对器官培养中胚胎和新生大鼠睾丸的影响。
DOI：
10.1002/j.1939-4640.2003.tb02723.x
复制
发表时间：
2003
影响因子：
--
通讯作者：
Kim,KwanHee
中科院分区：
文献类型：
--
作者：
Li,Hui;Kim,KwanHee
成年动物和人类接触乙醇会对雄性生殖功能产生显着的抑制作用，但乙醇接触对胚胎和产后早期睾丸发育的影响尚不清楚。目前的研究利用器官培养技术研究了乙醇对胚胎和新生儿睾丸发育的影响。在胚胎第 13 天 (E13) 睾丸器官培养中，乙醇对睾丸索的形成、支持细胞中苗勒氏管抑制物质 (MIS) 的表达或生殖母细胞的数量没有影响。同样，在乙醇处理的胚胎第 18 天 (E18) 睾丸中，生殖细胞的数量以及 GATA-4 和 MIS 的表达与对照睾丸相似。相比之下，在出生后第 3 天 (P3) 睾丸中，浓度为 150 和 200 mM 的乙醇显着减少了生殖细胞的数量，而不影响支持细胞中 GATA-4 和 MIS 的表达。该效应被证明是由于生殖细胞凋亡增强所致。此外，随着乙醇治疗剂量的增加，RARα 的核定位增加表明，乙醇异常激活了视黄酸受体 α (RARα)。这些观察结果表明，乙醇对睾丸的影响在胚胎和新生儿睾丸发育的不同阶段有所不同。此外，生殖细胞可能是乙醇对产后早期睾丸作用的主要目标。
Ethanol exposure in adult animals and humans has shown to elicit significant inhibitory effects on the function of male reproduction, but consequences of ethanol exposure on the embryonic and early postnatal testis development are not known. The current study investigated the effect of ethanol on embryonic and neonatal testis development using an organ culture technique. In embryonic day 13 (E13) testis organ cultures, ethanol had no effect on the testicular cord formation, the expression of Müllerian‐inhibiting substance (MIS) in Sertoli cells or the number of gonocytes. Similarly, in the ethanol‐treated embryonic day 18 (E18) testes, both the number of gonocytes and the expression of GATA‐4 and MIS were similar to those from the control testes. In contrast, in postnatal day 3 (P3) testes, ethanol at concentrations of 150 and 200 mM significantly decreased the number of gonocytes without affecting the expression of GATA‐4 and MIS in Sertoli cells. This effect was shown to be resulting from the enhanced apoptosis of gonocytes. In addition, ethanol abnormally activated retinoic acid receptor alpha (RARα), as indicated by increased nuclear localization of RARα with increasing doses of ethanol treatment. These observations suggest that the effect of ethanol on testis varies at different stages during embryonic and neonatal testis development. Furthermore, germ cells may be the main target for the action of ethanol on the early postnatal testis.