Analysis of the in vivo assembly pathway of eukaryotic 40S ribosomal proteins
Analysis of the in vivo assembly pathway of eukaryotic 40S ribosomal proteins
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DOI：
10.1016/j.molcel.2007.09.029
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发表时间：
2007-11-09
期刊：
影响因子：
16
通讯作者：
Milkereit, Philipp
中科院分区：
文献类型：
--
作者：
Ferreira-Cerca, Sebastien;Poell, Gisela;Milkereit, Philipp
在真核生物中，由4个核糖体RNA(RRNAs)和大约80个核糖体蛋白(r蛋白)在体内形成的两个核糖体亚基涉及150多个非核糖体蛋白和大约100个非编码小RNA。它在时间和空间上组织在不同的细胞室中：核仁、核质和细胞质。在这里，我们提出了一种方法来分析真核细胞中小核糖体亚单位(SSU)的r蛋白如何在体内与rRNA组装。我们的结果表明，真核r蛋白组装成SSU不同结构部分的关键方面与其原核对应蛋白的体外组装途径相似。我们观察到，真核细胞SSU主体而不是SSU头部稳定组装中间体的建立与早期的rRNA加工事件密切相关。头部组装中间体的形成控制着SSU的有效核输出和细胞质前rRNA成熟步骤。
In eukaryotes, in vivo formation of the two ribosomal subunits from four ribosomal RNAs (rRNAs) and approximately 80 ribosomal proteins (r-proteins) involves more than 150 non-ribosomal proteins and around 100 small non-coding RNAs. It is temporally and spatially organized within different cellular compartments: the nucleolus, the nucleoplasm, and the cytoplasm. Here, we present a way to analyze how eukaryotic r-proteins of the small ribosomal subunit (SSU) assemble in vivo with rRNA. Our results show that key aspects of the assembly of eukaryotic r-proteins into distinct structural parts of the SSU are similar to the in vitro assembly pathway of their prokaryotic counterparts. We observe that the establishment of a stable assembly intermediate of the eukaryotic SSU body, but not of the SSU head, is closely linked to early rRNA processing events. The formation of assembly intermediates of the head controls efficient nuclear export of the SSU and cytoplasmic pre-rRNA maturation steps.