Luminescence of aequorin is triggered by the binding of two calcium ions.
Luminescence of aequorin is triggered by the binding of two calcium ions.
复制标题
水母发光蛋白的发光是由两个钙离子的结合触发的。
DOI：
--
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
O. Shimomura
中科院分区：
文献类型：
--
作者：
O. Shimomura
发光蛋白水母发光蛋白能在微量Ca 2+存在下发光，是研究细胞内钙离子的有用指示剂。水母发光蛋白的一级结构表明存在三个Ca（2+）-结合位点，而发光强度与Ca 2+浓度的对数-对数图给出的斜率范围为2至3，这取决于所使用的条件，表明在发光反应中涉及两个或三个Ca 2+离子。在解释水母发光蛋白的测定结果时，关于Ca 2+化学计量的准确信息至关重要。这项研究清楚地表明，水母发光蛋白的发光是由两个Ca 2+离子的结合触发，基于用Ca 2+滴定水母发光蛋白的结果。
The photoprotein aequorin, capable of emitting light in the presence of a trace amount of Ca2+, is a useful indicator for studying intracellular calcium. The primary structure of aequorin indicated the presence of three Ca(2+)-binding sites, whereas log-log plots of the luminescence intensity versus Ca2+ concentration gave slopes ranging from 2 to 3 depending on the conditions used, suggesting the involvement of two or three Ca2+ ions in the luminescence reaction. Accurate information on the stoichiometry of Ca2+ is essential in interpreting the assay results obtained with aequorin. This study clearly shows that aequorin luminescence is triggered by the binding of two Ca2+ ions, based on the results of titrating aequorin with Ca2+.