Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells.
Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells.
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DOI：
10.1083/jcb.200011013
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发表时间：
2001-07-09
期刊：
影响因子：
--
通讯作者：
Yumura S
中科院分区：
文献类型：
--
作者：
Yumura S
肌球蛋白II是收缩环的主要成分。为了检查肌球蛋白II是否在收缩环中翻转，通过激光照射局部漂白在网骨藻细胞中表达的GFP-肌球蛋白II的荧光，并监测恢复。荧光恢复半衰期为7.01 ± 2.62 s。这种恢复不是由肌球蛋白II从非漂白区的侧向运动引起的，而是由与内质肌球蛋白II的交换引起的。在表达GFP-3ALA肌球蛋白II的细胞中进行类似的实验，其中三个可磷酸化的苏氨酸残基被丙氨酸残基取代。在这种情况下，在相当的时间范围内未检测到回收率。这些结果表明，肌球蛋白II在收缩环进行动态周转通过其重链磷酸化。由于GFP-3ALA肌球蛋白II没有显示恢复，它作为一个有用的标记肌球蛋白II运动，这使我们能够证明皮质流向赤道肌球蛋白II的第一次。因此，皮质流伴随着收缩环形成过程中肌球蛋白II的动态交换。
Myosin II is a major component of a contractile ring. To examine if myosin II turns over in contractile rings, fluorescence of GFP–myosin II expressed in Dictyostelium cells was bleached locally by laser illumination, and the recovery was monitored. The fluorescence recovered with a half time of 7.01 ± 2.62 s. This recovery was not caused by lateral movement of myosin II from the nonbleached area, but by an exchange with endoplasmic myosin II. Similar experiments were performed in cells expressing GFP–3ALA myosin II, of which three phosphorylatable threonine residues were replaced with alanine residues. In this case, recovery was not detected within a comparable time range. These results indicate that myosin II in the contractile ring performs dynamic turnover via its heavy chain phosphorylation. Because GFP–3ALA myosin II did not show the recovery, it served as a useful marker of myosin II movement, which enabled us to demonstrate cortical flow of myosin II toward the equator for the first time. Thus, cortical flow accompanies the dynamic exchange of myosin II during the formation of contractile rings.
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由 myo2 基因编码的 II 型肌球蛋白重链在粟酒裂殖酵母胞质分裂过程中组成收缩环。
DOI：
10.1083/jcb.137.6.1309
发表时间：
1997-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kitayama C;Sugimoto A;Yamamoto M
通讯作者：
Yamamoto M
影响因子：
3.3
作者：
Sabry, JH;Moores, SL;Spudich, JA
通讯作者：
Spudich, JA
影响因子：
56.9
作者：
KNECHT, DA;LOOMIS, WF
通讯作者：
LOOMIS, WF
影响因子：
64.8
作者：
Dickson, RM;Cubitt, AB;Moerner, WE
通讯作者：
Moerner, WE
影响因子：
9.2
作者：
Rizzuto, R;Brini, M;Pozzan, T
通讯作者：
Pozzan, T