RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids
RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids
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DOI：
10.1038/emboj.2012.274
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发表时间：
2012-11-05
期刊：
影响因子：
11.4
通讯作者：
Knolle, Percy A.
中科院分区：
文献类型：
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作者：
Abdullah, Zeinab;Schlee, Martin;Knolle, Percy A.
抗单核细胞增生李斯特菌感染的免疫力不是通过与被杀死的李斯特菌接触而从先天免疫刺激中获得的，而是需要活的李斯特菌进入感染细胞的胞质溶胶。它仍然是不明确的如何活李斯特菌的免疫感应发生。在这里，我们报告说，有效的胞质免疫传感需要从活李斯特菌获得的核酸进入受感染细胞的细胞质。我们发现李斯特菌释放核酸，并且这种分泌的细菌RNA/DNA被胞质传感器RIG-I、MDA 5和STING识别，从而触发干扰素β产生。分泌的李斯特菌核酸还引起RIG-I依赖性IL-1 β产生和炎性小体活化。信号分子CARD 9响应于分泌的核酸而促进IL-1 β的产生。总之，RIG-I对分泌的细菌核酸的胞质识别为活细菌有效诱导免疫提供了机制解释。The EMBO Journal（2012）31，4153-4164. doi：10.1038/daj.2012.274; 2012年10月12日在线发布
Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill-defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria to the cytoplasm of infected cells. We found that Listeria released nucleic acids and that such secreted bacterial RNA/DNA was recognized by the cytosolic sensors RIG-I, MDA5 and STING thereby triggering interferon beta production. Secreted Listeria nucleic acids also caused RIG-I-dependent IL-1 beta-production and inflammasome activation. The signalling molecule CARD9 contributed to IL-1 beta production in response to secreted nucleic acids. In conclusion, cytosolic recognition of secreted bacterial nucleic acids by RIG-I provides a mechanistic explanation for efficient induction of immunity by live bacteria. The EMBO Journal (2012) 31, 4153-4164. doi: 10.1038/emboj.2012.274; Published online 12 October 2012