Separation Behavior of Biological Constituents Having cis-Diol Groups through Interactions with Phenylboronic Acid Sites Introduced on the Inner Wall of a Fused-Silica Capillary
Separation Behavior of Biological Constituents Having cis-Diol Groups through Interactions with Phenylboronic Acid Sites Introduced on the Inner Wall of a Fused-Silica Capillary
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具有顺式二醇基团的生物成分通过与熔融石英毛细管内壁上引入的苯基硼酸位点相互作用的分离行为
DOI：
10.1246/bcsj.71.2831
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发表时间：
1998
影响因子：
3.1
通讯作者：
K. Kondo
中科院分区：
文献类型：
--
作者：
K. Tsukagoshi;M. Hashimoto;M. Otsuka;R. Nakajima;K. Kondo
在熔融石英毛细管内壁上引入苯基硼酸位点，通过3-丙烯酰胺基苯基硼酸的聚合反应后，由硅烷偶联剂活化的壁。从分离化学的不同角度考察了苯硼酸修饰毛细管。具有顺式二醇基团的核苷主要用作模型样品。苯硼酸修饰的毛细管成功地分离了核苷的混合物，例如，腺苷和脱氧腺苷的混合物，在毛细管电泳。分离是基于顺式二醇基团和毛细管壁上的苯基硼酸位点之间的相互作用。硼酸盐迁移缓冲液与改性毛细管配合使用可提高分离度。用改进的毛细管电泳法，在β-环糊精共存的条件下，D-和L-葡萄糖混合物也得到了分离。此外，还用显色法对核苷类化合物进行了分离，获得了满意的结果。
Phenylboronic acid sites were introduced onto the inner wall of a fused-silica capillary through the polymerization of 3-acrylamidophenylboronic acid after activating the wall by a silane coupling reagent. The phenylboronic acid-modified capillary was examined from various viewpoints of separation chemistry. Nucleosides having cis-diol groups were mainly used as model samples. The phenylboronic acid-modified capillary successfully separated a mixture of nucleosides, for example, an adenosine and deoxyadenosine mixture, in capillary electrophoresis. The separation was based on an interaction between the cis-diol groups and the phenylboronic acid sites on the capillary wall. The cooperation of borate migration buffer with the modified capillary could improve the resolution. The separation of a D- and L-glucoses mixture was also indicated with the coexistence of β-cyclodextrin in electrophoresis using a modified capillary. Furthermore, mixtures of nucleosides were separated with satisfactory results by chrom...