Cyclooxygenase-2 expression is induced during human megakaryopoiesis and characterizes newly formed platelets
Cyclooxygenase-2 expression is induced during human megakaryopoiesis and characterizes newly formed platelets
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DOI：
10.1073/pnas.112202999
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发表时间：
2002-05-28
影响因子：
11.1
通讯作者：
Patrono, C
中科院分区：
文献类型：
--
作者：
Rocca, B;Secchiero, P;Patrono, C
环氧合酶（考克斯）-1或-2和前列腺素（PG）脱氢酶催化各种PG和血栓烷（TX）A的形成（2）。我们研究了考克斯-1和-2在人巨核细胞生成过程中的表达和活性。我们分析了来自骨髓活检的巨核细胞和来自培养中血小板生成素处理的CD 34（+）造血祖细胞的巨核细胞。血小板取自健康供者和因免疫性血小板减少症或外周血干细胞移植而血小板再生率高的患者。通过免疫细胞化学，考克斯-1在CD 34（+）细胞和巨核细胞中的每个成熟阶段都被观察到，而考克斯-2在培养6天后被诱导，并且在成熟的巨核细胞中仍然可检测到。CD 34+细胞合成的PGE（2）比TXB 2多（214 +/- 50 vs. 30 +/- 10 pg/10（6）cells），而成熟巨核细胞则相反（TXB 2 8，440 +/-2，500 vs. PGE（2）906 +/- 161 pg/10（6）cells）。通过免疫组化染色，观察到考克斯-2在
Cyclooxygenase (COX)-1 or -2 and prostaglandin (PG) synthases catalyze the formation of various PGs and thromboxane (TX) A(2). We have investigated the expression and activity of COX-1 and -2 during human megakaryocytopoiesis. We analyzed megakaryocytes from bone marrow biopsies and derived from thrombopoietin-treated CD34(+) hemopoietic progenitor cells in culture. Platelets were obtained from healthy donors and patients with high platelet regeneration because of immune thrombocytopenia or peripheral blood stem cell transplantation. By immunocytochemistry, COX-1 was observed in CD34(+) cells and in megakaryocytes at each stage of maturation, whereas COX-2 was induced after 6 days of culture, and remained detectable in mature megakaryocytes. CD34+ cells synthesized more PGE(2) than TXB2 (214 +/- 50 vs. 30 +/- 10 pg/10(6) cells), whereas the reverse was true in mature megakaryocytes (TXB2 8,440 +/- 2,500 vs. PGE(2) 906 +/- 161 pg/10(6) cells). By immunostaining, COX-2 was observed in