4'-THIO-RNA - SYNTHESIS OF MIXED BASE 4'-THIO-OLIGORIBONUCLEOTIDES, NUCLEASE RESISTANCE, AND BASE-PAIRING PROPERTIES WITH COMPLEMENTARY SINGLE AND DOUBLE-STRAND
4'-THIO-RNA - SYNTHESIS OF MIXED BASE 4'-THIO-OLIGORIBONUCLEOTIDES, NUCLEASE RESISTANCE, AND BASE-PAIRING PROPERTIES WITH COMPLEMENTARY SINGLE AND DOUBLE-STRAND
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DOI：
10.1089/ard.1995.5.167
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发表时间：
1995-09-01
期刊：
影响因子：
--
通讯作者：
IMBACH, JL
中科院分区：
文献类型：
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作者：
LEYDIER, C;BELLON, L;IMBACH, JL
通过亚磷酰胺固相载体法合成了含有混合碱基序列的4 ′-硫代-β-D-寡核苷酸（12 mer和16 mer）。与野生型RNA相比，这些RNA类似物显示出非常好的核酸酶抗性。此外，4 ′-硫代-β-D-寡核苷酸显示出与互补DNA或RNA链杂交形成双链体或与DNA发夹杂交形成三螺旋。4 '-硫代-RNA与其互补RNA链的结合比与其互补DNA链的结合更紧密。4'-硫代-RNA：RNA双链体与2 '-O-甲基-RNA：RNA双链体一样稳定。然而，4 ′-硫代-RNA形成4 ′-硫代-RNA：DNA：DNA三聚体，其稳定性类似于所有野生型DNA的相应三聚体。
4'-Thio-beta-D-oligoribonucleotides (12 mer and 16 mer) containing a mixed base sequence were synthesized via the phosphoramidite solid support approach, These RNA analogs showed very good nuclease resistance as compared with wild-type RNA, Furthermore, 4'-thio-beta-D-oligoribonucleotides were shown to hybridize with a complementary DNA or RNA strand to form a duplex or with a DNA hairpin to form a triple helix. 4'-Thio-RNA binds more tightly to its complementary RNA strand than to its complementary DNA strand, A 4'-thio-RNA:RNA duplex is as stable as a 2'-O-methyl-RNA:RNA duplex. 4'-Thio-RNA, however, forms a 4'-thio-RNA:DNA:DNA tripler with a stability similar to the corresponding tripler with all wild-type DNA.