Anticataractogenic effect of betaine in chick embryo hydrocortisone-induced cataract model
Anticataractogenic effect of betaine in chick embryo hydrocortisone-induced cataract model
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DOI：
10.4103/ijmr.ijmr_50_18
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发表时间：
2019-10-01
影响因子：
4.2
通讯作者：
Guzel, Hilal
中科院分区：
文献类型：
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作者：
Duman, Resat;Ertekin, Tolga;Guzel, Hilal
背景与目的：白内障是世界上致盲的主要原因之一。本研究旨在探讨甜菜碱对氢化可的松(HC)诱导的鸡胚性白内障模型的抑制作用。方法：将60枚受精卵随机分为6组，每组10枚：1组为HC组，3组为HC加不同剂量甜菜碱(HC/B 1.00、HC/B 0.50和HC/B 0.25组)，2组为非HC组(仅用磷酸盐缓冲液(PBS)或甜菜碱处理)。注射后取出胚胎晶状体，根据晶状体混浊程度分为5个阶段。结果：非HC处理组晶状体全部透明，而HC处理组90%的晶状体有白内障(4、5期)。各HC/B组晶状体混浊程度评分均显著低于HC组(2.4~3.5vs.4.4，P
Background & objectives: Cataract is one of the leading causes of blindness in the world. The aim of the present study was to investigate anticataractogenic effect of betaine in chick embryo hydrocortisone (HC)-induced cataract model.Methods: The study included 60 fertilized eggs divided into six groups each having 10 eggs: one group treated with only HC (HC group); three treated with both HC and different doses of betaine (HC/B 1.00, HC/B 0.50 and HC/B 0.25 groups) and two non-HC groups treated with only phosphate-buffered saline (PBS group) or betaine (B group). After the injections, lenses of the embryos were removed and classified into five stages according to the lens opacification. The amounts of reduced glutathione (GSH) in the removed lenses were measured.Results: All the lenses in non-HC-treated groups were clear, whereas in the HC-treated group, 90 per cent of the lenses had cataract (stages 4 and 5). The mean score of lens opacity was significantly lower in all HC/B groups compared to HC group (2.4-3.5 vs. 4.4, P