SOME EFFECTS OF HYPOPHYSECTOMY AND PARATHYROID EXTRACT ON BONE MATRIX BIOSYNTHESIS.
SOME EFFECTS OF HYPOPHYSECTOMY AND PARATHYROID EXTRACT ON BONE MATRIX BIOSYNTHESIS.
复制标题
垂体切除术和甲状旁腺提取物对骨基质生物合成的一些影响。
作者：
C. Johnston;W. P. Deiss
测量了垂体切除术对实验动物颅骨和软骨中同位素体外掺入羟脯氨酸和己糖胺的影响。垂体切除术后，软骨中羟脯氨酸和己糖胺的比活性降低，而颅骨中两者的比活性均增加。因此，软骨和膜骨对垂体切除术的反应不同。当以每 100 mg 骨合成的 /xmoles 测量 6 小时孵育期间羟脯氨酸的合成时，发现垂体切除动物和对照动物的骨中羟脯氨酸的合成基本相同。有人认为颅骨的膜骨主要通过细胞分裂生长；因此，来自垂体切除动物或对照动物的测量骨量可以含有相同数量的细胞并合成等量的羟脯氨酸。垂体切除动物的骨基质羟脯氨酸的比活性可能会增加，因为...
The effect of hypophysectomy on in vitro incorporation of isotope into hydroxyproline and hexosamine of calvaria and cartilage of experimental animals has been measured. Following hypophysectomy, the specific activity of hydroxyproline and hexosamine of cartilage was reduced, while the specific activity of both was increased in calvaria. Thus, cartilage and membranous bone respond differently to hypophysectomy. When synthesis of hydroxyproline during a 6-hr incubation was measured as /xmoles synthesized per 100 mg bone, it was found to be essentially the same in bone from hypophysectomized and control animals. It is suggested that the membranous bone of the calvarium grows primarily by cell division; consequently, a measured quantity of bone from either hypophysectomized or control animals could contain an equal number of cells and synthesize an equal quantity of hydroxyproline. The specific activity of matrix hydroxyproline of bone from hypophysectomized animals was probably increased because of a reduce...