IMMUNITY AFTER TREATMENT OF HUMAN SCHISTOSOMIASIS - ASSOCIATION BETWEEN IGE ANTIBODIES TO ADULT WORM ANTIGENS AND RESISTANCE TO REINFECTION
IMMUNITY AFTER TREATMENT OF HUMAN SCHISTOSOMIASIS - ASSOCIATION BETWEEN IGE ANTIBODIES TO ADULT WORM ANTIGENS AND RESISTANCE TO REINFECTION
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DOI：
10.1002/eji.1830220622
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发表时间：
1992-06-01
影响因子：
5.4
通讯作者：
STURROCK, RF
中科院分区：
文献类型：
--
作者：
DUNNE, DW;BUTTERWORTH, AE;STURROCK, RF
先前对学龄儿童的研究表明，曼氏血吸虫感染化疗后对再感染的耐药性随着年龄的增长而缓慢发展，并表明不适当的（“阻断”）抗体反应阻止了幼儿免疫的表达。本研究旨在通过对居住在肯尼亚马查科斯区一个流行地区的151名曼索尼血吸虫感染者进行血清学研究，进一步调查这种保护性反应的性质。治疗前血液样本中针对各种抗原的抗体水平与先前感染的强度有关；治疗后6个月采集的血液样本中的抗体与随后30个月的累积再感染率有关。针对成虫抗原制备的IgE与年龄呈正相关，与再感染呈负相关。相反，针对其他生命周期阶段的IgE抗体要么没有关系，要么呈负相关。此外，针对成虫抗原的其他同型抗体与再感染没有相关性。与IgE的相关性可以在不同的成虫制剂中得到证明，包括经高碘酸盐处理的推定碳水化合物表位耗尽的制剂。对于完整的和含高碘酸盐的制剂，对小于或等于16岁儿童的结果进行的多元回归分析表明，在考虑年龄因素后，IgE效应存在，尽管在先前的学龄儿童研究组中未观察到这种效应。对成虫制备物进行Western blot分析，发现了一组有限的IgE识别抗原，其中22 kDa的抗原最为突出。针对该抗原的IgE的定性存在也可以证明与缺乏随后的再感染有关。
Previous studies in school children have demonstrated the slow development with age of resistance to reinfection after chemotherapy of Schistosoma mansoni infections, and have indicated that inappropriate ("blocking") antibody responses prevent the expression of immunity in young children. The present study was designed to investigate further the nature of the protective responses, by serological studies on a group of 151 S. mansoni-infected individuals resident in an endemic area in Machakos District, Kenya. Antibody levels against various antigens in blood samples before treatment were related to intensity of previous infections; antibodies in blood samples taken 6 months after treatment were related to cumulative reinfection rates over the following 30 months.IgE against an adult-worm antigen preparation correlated positively with age and negatively with reinfection. In contrast, IgE antibodies against other life-cycle stages showed either no relationship or the reverse correlation. Furthermore, antibodies of other isotypes against adult-worm antigens showed no correlations with reinfection.The correlation with IgE could be demonstrated for different preparations of adult worms, including a periodate-treated preparation presumptively depleted of carbohydrate epitopes. For both the intact and the periodate-treated preparations, multiple regression analysis of the results for children less-than-or-equal-to 16 years old demonstrated an IgE effect after allowing for age, although this effect was not observed in a previously studied group of school children.Western blot analysis of the adult-worm preparation revealed a limited set of antigens recognized by IgE, among which an antigen of 22 kDa was prominent. The qualitative presence of IgE against this antigen could also be shown to be related to a lack of subsequent reinfection.