THE FIFTH (ELECTROPHORETICALLY SLOWEST) SERUM LACTIC DEHYDROGENASE AS AN INDEX OF LIVER INJURY
THE FIFTH (ELECTROPHORETICALLY SLOWEST) SERUM LACTIC DEHYDROGENASE AS AN INDEX OF LIVER INJURY
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第五（电泳最慢）血清乳酸脱氢酶作为肝损伤的指标
DOI：
10.1111/j.1749-6632.1961.tb35583.x
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发表时间：
1961
影响因子：
5.2
通讯作者：
Y. Maercke
中科院分区：
文献类型：
--
作者：
R. Wieme;Y. Maercke
用不同的电泳技术将正常人血清乳酸脱氢酶活性（LDH）分为5个组分。在本文中，我们将考虑在pH为8.4时迁移最慢的LDH部分的意义，并且根据常规电泳实践，我们将此部分称为LDH5。在下文中，我们描述了电泳和酶检测技术（“酶电泳”）在我们的研究中使用。在《关于人血清LDH5的数据综述C》中，我们回顾了已经发表的关于LDH5的发现，在《新的实验调查》中，我们报告了在大鼠和猴子身上进行的新实验。结果表明，血清LDH5水平与肝细胞坏死转换之间存在直接的实验证据。
Total lactic dehydrogenase activity (LDH) of normal human serum has been separated by different electrophoretic techniques into 5 fractions. In this paper we shall consider the significance of that LDH fraction that, a t pH 8.4, migrates most slowly, and to this fraction we shall refer, according to conventional electrophoretic practice, as LDH5. Under Enzymoelectrophoresis, below, we describe the technique of electrophoresis and of enzyme detection (“enzymoelectrophoresis”) used in our studies. Under Review of Data C‘oncerning Human Serum LDHS.5 we review findings concerning LDH5 that already have been published, and, under New Experimental Investigations we report on new experiments done on rats and monkeys. I t is concluded that direct experimental proof has been given of the relation between the serum level of LDH5 and the necrotic turnover occurring in liver cells.