Characterisation and transfer of antibiotic resistance genes from enterococci isolated from food
Characterisation and transfer of antibiotic resistance genes from enterococci isolated from food
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DOI：
10.1016/j.syapm.2006.02.004
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发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Franz, Charles M. A. P.
中科院分区：
文献类型：
--
作者：
Hummel, Anja;Holzapfel, Wilhelm H.;Franz, Charles M. A. P.
38种耐抗生素粪肠球菌和粪肠球菌对抗生素四环素[tet(M)、tet(O)、tet(S)、tet(K)和tet(L)]、红霉素(ermA,B,C; mefA,E; msrA/B;和ereA,B)和氯霉素(cat)耐药性的遗传决定因素对食物中的菌株进行了表征。此外，还使用过滤交配测定评估了抗性基因的可转移性。 tet(L) 决定簇在四环素耐药肠球菌中最常见（占 94% 的菌株），其次是 tet(M) 基因，占 63.0% 的菌株。 56.0%的耐药菌株出现Tet(K)，而tet(O)和tet(S)基因未检出。 81.3%的四环素抗性菌株中存在Tn916-1545转座子家族的整合酶基因，表明抗性基因可能通过转座子转移。所有氯霉素耐药菌株均携带猫基因。 81.8%的红霉素耐药菌株携带ermB基因。 21 株红霉素抗性菌株中，有 2 株（9.5%）不含 ermA、B、C、ereA、B 和 mphA 基因，但含有编码红霉素外排泵的 msrC 基因，这通过 PCR 扩增子测序得到证实。此外，所有粪肠球菌菌株均含有msrC基因，但粪肠球菌菌株均不含。抗生素抗性遗传决定因素的转移只能在一项过滤器交配实验中得到证实，其中tet(M)和tet(L)基因均从粪肠球菌FAIR-E 315转移到粪肠球菌OG1X受体菌株。我们的结果表明，肠球菌中存在各种类型的抗性基因以及与可转移抗性相关的转座子整合酶基因，表明食品环境中基因转移的潜力。 (c) 2006 年爱思唯尔有限公司。版权所有。
The genetic determinants responsible for the resistances against the antibiotics tetracycline [tet(M), tet(O), tet(S), tet(K) and tet(L)], erythromycin (ermA,B,C; mefA,E; msrA/B; and ereA,B) and chloramphenicol (cat) of 38 antibiotic-resistant Enterococcus faecium and Enterococcus faecalis strains from food were characterised. In addition, the transferability of resistance genes was also assessed using filter mating assays. The tet(L) determinant was the most commonly detected among tetracycline-resistant enterococci (94% of the strains), followed by the tet(M) gene, which occurred in 63.0% of the strains. Tet(K) occurred in 56.0% of the resistant strains, while genes for tet(O) and tet(S) could not be detected. The integrase gene of the Tn916-1545 family of transposons was present in 81.3% of the tetracycline resistant strains, indicating that resistance genes might be transferable by transposons. All chloramphenicol-resistant strains carried a cat gene. 81.8% of the erythromycin-resistant strains carried the ermB gene. Two (9.5%) of the 21 erythromycin-resistant strains, which did not contain ermA,B,C, ereA,B and mphA genes harboured the msrC gene encoding an erythromycin efflux pump, which was confirmed by sequencing the PCR amplicon. In addition, all E. faecium strains contained the msrC gene, but none of the E. faecalis strains. Transfer of the genetic determinants for antibiotic resistance could only be demonstrated in one filter mating experiment, where both the tet(M) and tet(L) genes were transferred from E. faecalis FAIR-E 315 to the E. faecalis OG1X recipient strain. Our results show the presence of various types of resistance genes as well as transposon integrase genes associated with transferable resistances in enterococci, indicating a potential for gene transfer in the food environment. (c) 2006 Elsevier GmbH. All rights reserved.