Proteomic pattern of implantative human endometrial fluid in in vitro fertilization cycles
Proteomic pattern of implantative human endometrial fluid in in vitro fertilization cycles
复制标题
体外受精周期中植入性人子宫内膜液的蛋白质组学模式
DOI：
--
复制
发表时间：
2018
影响因子：
2.6
通讯作者：
D. Nagore
中科院分区：
文献类型：
--
作者：
R. Matorras;S. Quevedo;B. Corral;B. Prieto;A. Exposito;R. Mendoza;A. Rabanal;M. Díaz;M. Ferrando;F. Elortza;A. Ametzazurra;D. Nagore
目的评估子宫内膜吸出液 (EFA) 中是否存在预测着床的蛋白质。方法研究对象包括 285 名接受胚胎移植 (ET) 的女性。在 ET 前立即进行子宫内膜液体抽吸。对 33 例成功妊娠的病例和 33 例未妊娠的病例进行了 EFA 蛋白质组学分析结果的比较。通过二维电泳和质谱分析样品。通过 ELISA 研究血液样本的妊娠率，并将母体并发症与拒绝抽吸的女性进行比较。结果我们发现受孕周期 EFA 中有 23 种蛋白质差异表达：4 种上调蛋白质和 19 种下调蛋白质 (FC = 0.31 0.78)（其中包括精氨酸酶 1、肌动蛋白 B、PARK-7、cofilin-1、stathmin、annexin-2 和CAPZB）。在 EFA 中差异表达的五种研究蛋白质中，血清中没有差异表达。抽吸手术对妊娠率没有影响。没有报告母体并发症。结论我们发现植入周期中的蛋白质谱非常不同，大多数蛋白质被下调。这可能反映了不同的子宫内膜功能状态，更有利于着床。 EFA 蛋白质组分析可能是规划 ET 策略的有用工具。
PurposeTo assess whether there are proteins in endometrial fluid aspirate (EFA) that predict implantation.MethodsThe population under study consisted of 285 women undergoing embryo transfer (ET). Endometrial fluid aspiration was performed immediately before ET. Results of proteomic analysis of EFA were compared between 33 cases who achieved pregnancy and 33 who did not. Samples were analysed by 2D electrophoresis and mass spectrometry. Blood samples were studied by ELISA Pregnancy rates and maternal complications were compared to those in women refusing aspiration.ResultsWe found 23 proteins differentially expressed in the EFA in conception cycles: 4 up-regulated proteins and 19 down-regulated (FC = 0.31 0.78) (among others, arginase-1, actin B, PARK-7, cofilin-1, stathmin, annexin-2 and CAPZB). Among the five studied proteins that were differentially expressed in EFA, none was differentially expressed in serum. The aspiration procedure had no impact on pregnancy rate. No maternal complications were reported.ConclusionsWe found a very different protein profile in implantative cycles, the majority of proteins being down-regulated. This probably reflects a different endometrial functional status, more favourable to implantation. EFA proteomic analysis could be a useful tool in the planning ET strategies.