Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants.
Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants.
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中东呼吸道综合征冠状病毒内宿内种群的特征是许多高频变体。
DOI：
10.1371/journal.pone.0146251
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Allen JE
中科院分区：
文献类型：
--
作者：
Borucki MK;Lao V;Hwang M;Gardner S;Adney D;Munster V;Bowen R;Allen JE
中东呼吸综合征冠状病毒(MERS-CoV)是一种与SARS病毒相关的人类新病原体。体外研究表明，这种病毒可能有广泛的宿主范围，这表明大流行的可能性增加。遗传和流行病学证据表明，骆驼是MERS病毒的宿主，但跨物种传播的机制尚不清楚，关于人类对感染的易感性仍存在许多问题。从实验中感染了人MERS-CoV分离物的三头骆驼的鼻部样本中获得了深度测序数据。基因组的大部分被覆盖，平均覆盖深度大于12,000倍。虽然在共有的序列中只检测到5个突变，但共鉴定出473个宿主内单核苷酸变异。这些变异中的许多都是高频存在的，可能会潜在地影响病毒的表型和检测分析的敏感性，这些检测分析针对这些区域进行引物或探针结合。
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding.