Cellular retinoic acid-binding protein and the role of retinoic acid in the development of the chick embryo.
Cellular retinoic acid-binding protein and the role of retinoic acid in the development of the chick embryo.
复制标题
细胞视黄酸结合蛋白和视黄酸在鸡胚发育中的作用。
DOI：
10.1016/0012-1606(89)90163-2
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Hirst,EA
中科院分区：
文献类型：
--
作者：
Maden,M;Ong,DE;Summerbell,D;Chytil,F;Hirst,EA
使用针对大鼠 CRABP 的亲和纯化抗体描述了细胞视黄酸结合蛋白 (CRABP) 在雏鸡发育的四个阶段中的分布。 CRABP 是视黄酸 (RA) 进入细胞时与其结合的蛋白质，可能反映这些细胞对 RA 的需求。我们发现了几个表现出高水平免疫反应性的离散细胞群。有些位于神经管中，例如连合神经元和背顶板。有些起源于神经嵴，如背根神经节、感觉轴突、交感神经节和肠神经节。其余的细胞群是某些结缔组织细胞、肢芽细胞和肌节细胞。这些结果表明，某些器官系统，特别是神经系统，在发育过程中需要 RA，它们可能进一步加深我们对类视黄醇对胚胎致畸作用的理解。
The distribution of cellular retinoic acid-binding protein (CRABP) in four stages of chick development is described using an affinity-purified antibody against rat CRABP. CRABP is the protein to which retinoic acid (RA) binds when it enters cells and may reflect the requirement of those cells for RA. We found several discrete cell populations which showed high levels of immunoreactivity. Some were in the neural tube such as the commissural neurons and the dorsal roof plate. Some were of neural crest origin such as the dorsal root ganglia, sensory axons, sympathetic ganglia, and enteric ganglia. The remaining populations were certain connective tissue cells, limb bud cells, and the myotome. These results suggest that certain organ systems, particularly the nervous system, have a requirement for RA during development and they may further our understanding of the teratogenic effects of retinoids on the embryo.