HERPES-SIMPLEX VIRUS-INFECTED CELLS CONTAIN A FUNCTION(S) THAT DESTABILIZES BOTH HOST AND VIRAL MESSENGER-RNAS
HERPES-SIMPLEX VIRUS-INFECTED CELLS CONTAIN A FUNCTION(S) THAT DESTABILIZES BOTH HOST AND VIRAL MESSENGER-RNAS
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DOI：
10.1073/pnas.84.7.1926
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
FRENKEL, N
中科院分区：
文献类型：
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作者：
KWONG, AD;FRENKEL, N
单纯疱疹病毒病毒粒子具有介导宿主蛋白合成关闭和宿主mRNA降解的功能。受此功能影响的病毒突变体（vhs突变体）先前已被导出。感染这些突变体的细胞表现出更稳定的宿主合成以及即时早期（. α。）病毒蛋白质。我们现在表明，与野生型病毒纯化病毒粒子相关的功能减少了宿主和。α的半衰期。mrna，而纯化的vhs-1突变病毒粒子缺乏这种活性。许多早期（β）和晚期（γ）的功能半衰期。-病毒mrna在突变病毒感染中也被延长。这些研究表明，野生型病毒粒子给细胞带来了一种不加区分地减少宿主和病毒转录物半衰期的功能，并且宿主的早期翻译关闭是这种功能的结果。这种功能可能促进在感染后转录上以不同的估计开启的基因组表达的快速转变。
The herpes simplex virus virion contains a function that mediate the shutoff of host-protein synthesis and the degradation of host mRNA. Viral mutants affected in this function (vhs mutants) have previously been derived. Cells infected with these mutants exhibit a more stable synthesis of host as well as the immediate early (.alpha.)-viral proteins. We now show that function associated with purified virions of the wild-type virus reduces the half-life of host and .alpha. mRNAs, whereas purified vhs-1 mutant virions lack this activity. The functional half-life of many early (.beta.)- and late (.gamma.)-viral mRNAs is also prolonged in mutant virus infections. These studies suggest that the wild-type virion brings into cells a function that indiscriminately reduces the half-life of both host and viral transcripts and that the early translational shutoff of the host is a consequence of this function. This function may facilitate rapid transitions in the expression of groups of genes that are transcriptionally turned on at different estimates after infection.