Hedgehog elicits signal transduction by means of a large complex containing the kinesin-related protein costal2
Hedgehog elicits signal transduction by means of a large complex containing the kinesin-related protein costal2
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DOI：
10.1016/s0092-8674(00)80331-1
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发表时间：
1997-07-25
期刊：
影响因子：
64.5
通讯作者：
Therond, PP
中科院分区：
文献类型：
--
作者：
Robbins, DJ;Nybakken, KE;Therond, PP
黑腹果蝇的hedgehog基因编码一种分泌蛋白（HH），该蛋白在细胞命运和模式中起着至关重要的作用。在这里，我们描述了一种蛋白质复合物，介导的信号转导HH。该复合物包括至少三种基因的产物：融合（蛋白丝氨酸/苏氨酸激酶），肘中断（转录因子）和costal 2（驱动蛋白样蛋白）。在没有HH的情况下，复合物以极大的亲和力与微管结合，但结合被HH逆转。FU的催化外结构域中的突变消除了该蛋白的生物学功能及其与COS 2的关联。我们的结论是，复杂的可能有利于信号从HH通过管理访问的肘中断蛋白质的核。
The hedgehog gene of Drosophila melanogaster encodes a secreted protein (HH) that plays a vital role in cell fate and patterning. Here we describe a protein complex that mediates signal transduction from HH. The complex includes the products of at least three genes:fused (a protein-serine/threonine kinase), cubitus interruptus (a transcription factor), and costal2 (a kinesin-like protein). The complex binds with great affinity to microtubules in the absence of HH, but binding is reversed by HH. Mutations in the extracatalytic domain of FU abolish both the biological function of the protein and its association with COS2. We conclude that the complex may facilitate signaling from HH by governing access of the cubitus interruptus protein to the nucleus.