Rat lung antioxidant enzyme activities and their specific proteins during hyperoxia.
Rat lung antioxidant enzyme activities and their specific proteins during hyperoxia.
复制标题
高氧期间大鼠肺抗氧化酶活性及其特异性蛋白。
DOI：
10.1152/jappl.1988.65.2.797
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Warshaw,JB
中科院分区：
文献类型：
--
作者：
Crouch,LS;Prough,RA;Kennedy,KA;Snyder,JB;Warshaw,JB
The hyperoxia-induced increases in the activity of lung glucose-6-phosphate dehydrogenase (G-6-P) and glutathione reductase (GR) after exposure of rats to greater than 97% O2 for 6 days were accompanied by equivalent increases in the amount of the respective immunoreactive proteins. Hyperoxia also increased lung glutathione (GSH) + oxidized glutathione (GSSG) content and the magnitude of this hyperoxic response of increased GSH + GSSG, G-6-P, and GR (maximal 1.3- to 1.8-fold) declined as a function of age during the first 3 wk of life. Fetal rat lung explants cultured 4 days in 95% O2 showed increased G-6-P and GR activity and increased levels of the specific proteins 1.5-fold those of explants at 2 days of culture. We conclude that the hyperoxic response of increased rat lung G-6-P and GR activity in vivo and in vitro involves not just alteration of enzyme activity but also specific increases in the proteins catalyzing the reactions.
