Establishment and characterization of an in vivo model for Epstein-Barr virus positive gastric carcinoma
Establishment and characterization of an in vivo model for Epstein-Barr virus positive gastric carcinoma
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DOI：
10.1002/jmv.20876
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发表时间：
2007-09-01
影响因子：
12.7
通讯作者：
Lee, Suk Kyeong
中科院分区：
文献类型：
--
作者：
Oh, Sang Taek;Cha, Jung-Ho;Lee, Suk Kyeong
由于缺乏合适的模型系统，有关EB病毒（EBV）在胃癌发生中作用的研究受到阻碍。SNU-719是一种自然感染EBV的胃癌细胞系。该细胞系在接种后约40-56天在裸鼠中形成肿瘤。SNU-719在体外也表现出低血清依赖性和锚定非依赖性生长。所发展的肿瘤表达EBERs、EBNA 1和LMP 2A，但不表达其他EBV潜伏基因。此外，Qp是活跃的，并且在肿瘤组织中观察到单克隆或双克隆EBV基因组。由于该细胞系具有EB病毒相关性胃癌的特征，因此可作为研究EB病毒相关性胃癌的发生机制和治疗方法的一个有用的体内系统。
Research regarding the role of the Epstein-Barr virus (EBV) in gastric carcinogenesis has been hampered by the absence of a suitable model system. SNU-719 is a gastric carcinoma cell line naturally infected with EBV. This cell line developed tumors in nude mice approximately 40-56 days after inoculation. SNU-719 also showed low serum dependency and anchorage independent growth in vitro. The developed tumors expressed EBERs, EBNA1, and LMP2A but not other EBV latent genes. Additionally, Qp was active and either mono- or bi-clonal EBV genome was observed in the tumor tissues. Because the developed tumors retained characteristics of EBV-associated gastric cancer,thiscell line could serve as a useful in vivo system to investigate the tumorigenesis mechanism and treatment methods for this type of tumor.