Sequential Assessment of Cell Cycle S Phase in Flow Cytometry: A Non-Isotopic Method to Measure Lymphocyte Activation In Vitro
Sequential Assessment of Cell Cycle S Phase in Flow Cytometry: A Non-Isotopic Method to Measure Lymphocyte Activation In Vitro
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流式细胞术中细胞周期 S 期的顺序评估：体外测量淋巴细胞活化的非同位素方法
DOI：
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发表时间：
1997
影响因子：
3.2
通讯作者：
G. Faure
中科院分区：
文献类型：
--
作者：
C. Kohler;M. Kolopp;A. De March‐Kennel;A. Barbaud;M. Béné;G. Faure
淋巴细胞增殖可以在体外由有丝分裂原或特异性抗原诱导，并且通常使用涉及氚化胸苷的同位素方法来测量。细胞增殖也可以通过基于细胞周期分析的流式细胞术技术通过测量DNA含量来分析。我们应用这种方法从113个人的淋巴细胞，评估淋巴细胞增殖刺激后，在体外由有丝分裂原（植物血凝素，PHA）或召回抗原（破伤风类毒素），使用动力学方法与四点连续测量的S和G2期超过6天的文化。PHA刺激后S期细胞的比例显著高于对照组，并且早在培养的第三天。激活与回忆抗原显着诱导增加S期细胞的比例到第六天。这些数据表明，S期的流式细胞术评估可能是测量体外淋巴细胞反应性的同位素方法的有用替代方法。
Lymphocyte multiplication can be induced in vitro by mitogens or specific antigens, and is usually measured using isotopic methods involving tritiated thymidine. Cellular proliferation can also be analyzed by flow cytometry techniques based on cell cycle analysis through the measurement of DNA content. We applied this method to lymphocytes from 113 individuals, to evaluate lymphocyte proliferation after stimulation in vitro by a mitogen (phytohaemagglutinin, PHA) or a recall antigen (tetanus toxoid), using a kinetic approach with four points sequential measurements of the S and G2 phases over six days of culture. The proportion of cells in S phase after PHA stimulation was significantly higher than in controls overall and as early as on day three of the culture. Activation with a recall antigen significantly induced increasing S phase cell proportions up to day six. These data suggest that flow cytometric assessment of the S phase could be a useful alternative to isotopic methods measuring lymphocyte reactivity in vitro.
麻疹疫苗失败时的细胞免疫：尽管重新接种后血清抗体产生有限，但仍显示出麻疹抗原特异性淋巴增殖反应。
DOI：
10.1093/infdis/172.6.1591
发表时间：
1995
期刊：
The Journal of infectious diseases
影响因子：
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作者：
Ward,BJ;Boulianne,N;Ratnam,S;Guiot,MC;Couillard,M;DeSerres,G
通讯作者：
DeSerres,G