Neonatal Phosphate Nutrition Alters in Vivo and in Vitro Satellite Cell Activity in Pigs
Neonatal Phosphate Nutrition Alters in Vivo and in Vitro Satellite Cell Activity in Pigs
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DOI：
10.3390/nu4060436
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发表时间：
2012-06-01
期刊：
影响因子：
5.9
通讯作者：
Stahl, Chad H.
中科院分区：
文献类型：
--
作者：
Alexander, Lindsey S.;Seabolt, Brynn S.;Stahl, Chad H.
卫星细胞的活动是出生后骨骼肌生长所必需的。严重的磷酸盐（PO 4）缺乏可以改变卫星细胞的活性，但新生儿PO 4营养对卫星细胞生物学的作用仍然不清楚。21头仔猪（1日龄，1.8 +/- 0.2 kg BW）配对饲喂12天的PO 4充足（0.9%总P）、超充足（1.2%总P）PO 4需要量或缺乏（0.7%总P）PO 4含量的流质饲料。每天记录体重，每6天采集一次血样。在第12天，猪口服给药BrdU和12小时后，卫星细胞分离。卫星细胞也在体外培养7天，以确定是否PO 4营养改变他们的能力，继续通过他们的肌源性谱系。日粮PO_4缺乏导致血清PO_4和甲状旁腺激素（PTH）浓度降低（P < 0.05），而超充足日粮PO_4提高饲料转化效率（P < 0.05）。体内卫星细胞增殖减少（P < 0.05），在PO 4缺乏的猪，这些细胞在体外表达的肌源性进展的标志物发生了改变。进一步的工作，以更好地了解卫星细胞的早期营养编程和强调早期PO 4营养对未来瘦肉生长潜力的潜在好处是必要的。
Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4) deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 +/- 0.2 kg BW) were pair-fed liquid diets that were either PO4 adequate (0.9% total P), supra-adequate (1.2% total P) in PO4 requirement or deficient (0.7% total P) in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured in vitro for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (P < 0.05) sera PO4 and parathyroid hormone (PTH) concentrations, while supra-adequate dietary PO4 improved (P < 0.05) feed conversion efficiency as compared to the PO4 adequate group. In vivo satellite cell proliferation was reduced (P < 0.05) among the PO4 deficient pigs, and these cells had altered in vitro expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.