Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF
Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF
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DOI：
10.1038/35054095
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发表时间：
2001-01-25
期刊：
影响因子：
64.8
通讯作者：
Brown, PO
中科院分区：
文献类型：
--
作者：
Iyer, VR;Horak, CE;Brown, PO
蛋白质与基因组DNA相互作用，使基因组栩栩如生。这些相互作用还定义了基因组的许多功能特征。 SBF和MBF是序列特异性转录因子，在酵母中细胞周期的G1/s转变过程中激活基因表达（1,2）。 SBF是SWI4和SWI6的异二聚体，MBF是MBP1和SWI6的异二聚体（参考1，3）。相关的SWI4和MBP1蛋白是相应因素的DNA结合成分，SWI6可能具有调节功能（4,5）。已经鉴定出少量的SBF和MBF靶基因（3,6-10）。在这里，我们通过使用DNA微阵列来定义体内SBF和MBF转录因子的基因组结合位点。除了先前特征的目标外，我们还确定了大约200个新的推定目标。我们的结果支持以下假设：SBF激活的基因主要参与萌芽，并且在膜和细胞壁生物合成中，而DNA复制和修复是MBF激活基因的主要功能（6,11）。这些因素的功能专业化可能为在有丝分裂细胞周期中通常在同步中发生的不同分子过程独立调节提供了一种机制。
Proteins interact with genomic DNA to bring the genome to life; and these interactions also define many functional features of the genome. SBF and MBF are sequence-specific transcription factors that activate gene expression during the G1/S transition of the cell cycle in yeast(1,2). SBF is a heterodimer of Swi4 and Swi6, and MBF is a heterodimer of Mbp1 and Swi6 (refs 1, 3). The related Swi4 and Mbp1 proteins are the DNA-binding components of the respective factors, and Swi6 may have a regulatory function(4,5). A small number of SBF and MBF target genes have been identified(3,6-10). Here we define the genomic binding sites of the SBF and MBF transcription factors in vivo, by using DNA microarrays. In addition to the previously characterized targets, we have identified about 200 new putative targets. Our results support the hypothesis that SBF activated genes are predominantly involved in budding, and in membrane and cell-wall biosynthesis, whereas DNA replication and repair are the dominant functions among MBF activated genes(6,11). The functional specialization of these factors may provide a mechanism for independent regulation of distinct molecular processes that normally occur in synchrony during the mitotic cell cycle.