Crystallization and preliminary X-ray analysis of two new crystal forms of calmodulin
Crystallization and preliminary X-ray analysis of two new crystal forms of calmodulin
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DOI：
10.1107/s0907444995011826
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Parkin, S
中科院分区：
文献类型：
--
作者：
Rupp, B;Marshak, DR;Parkin, S
报道了鸡钙调素的两种新晶型。空间群P1中的晶体(晶胞尺寸a=59.7，b=53.1，c=24.6埃，α=93.2，β=96.7，伽马=89.2度，Z=2)以细长的针状生长。含水率在密度方面的考量类似于50%。它们的衍射率类似于2.0埃，但给出了宽的多峰光斑轮廓。空间群P2(1)2(1)2(1)(晶胞尺寸a=32.2，b=56.0，c=67.3埃，Z=4)晶体呈片状生长，体积含水率接近30%。这些小晶体(类似于0.4×0.15×0.1 mm)的衍射率很好，接近1.4埃，在分辨率优于1.2埃的情况下观察到了一些明显的强度。
Two new crystal forms of calmodulin from Gallus gallus are reported. Crystals in space group P1 (cell dimensions a = 59.7, b = 53.1, c = 24.6 Angstrom, alpha = 93.2, beta = 96.7, gamma = 89.2 degrees and Z = 2), grow as long thin needles. Water content on density considerations is similar to 50%. They diffract to similar to 2.0 Angstrom, but give wide multiply peaked spot profiles. Crystals in space group P2(1)2(1)2(1) (cell dimensions a = 32.2, b = 56.0, c = 67.3 Angstrom and Z = 4), grow as clusters of thin tablets and contain similar to 30% water by volume. These small crystals (similar to 0.4 x 0.15 x 0.1 mm) diffracted well to similar to 1.4 Angstrom and some appreciable intensities were observed at resolutions better than 1.2 Angstrom.