Coupling Neuropeptide Levels to Structural Plasticity in Drosophila Clock Neurons
Coupling Neuropeptide Levels to Structural Plasticity in Drosophila Clock Neurons
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DOI：
10.1016/j.cub.2020.06.009
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发表时间：
2020-08-17
期刊：
影响因子：
9.2
通讯作者：
Fernanda Ceriani, Maria
中科院分区：
文献类型：
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作者：
Herrero, Anastasia;Yoshii, Taishi;Fernanda Ceriani, Maria
我们之前报道过，色素分散因子（PDF）神经元对果蝇的休息-活动周期的控制至关重要，其轴突投射会经历昼夜重塑，这种现象称为昼夜结构可塑性，轴突树枝化在白天表现出更高的复杂性，在晚上变得更简单，这种重塑涉及连通程度的变化。这种现象取决于腹外侧神经元 (LNvs) 以及神经胶质细胞中存在的时钟。在这项工作中，我们详细描述了 PDF 神经肽在一天中不同时间对结构可塑性的贡献。使用不同的遗传策略暂时限制其下调，我们证明即使是背侧原大脑 PDF 循环的细微改变也与受损的重塑相关，强调了其与特征性早晨传播的相关性；小 LNvs (sLNvs) 和大 LNvs (ILNvs) 释放的 PDF 有助于该过程。此外，强制去极化招募活动依赖性机制仅在夜间介导生长，克服了时钟对膜兴奋性的限制。有趣的是，末端重塑的主动过程需要 PDF 受体 (PDFR) 信号通过环核苷酸门控通道离子通道亚基 A (CNGA) 局部发挥作用。因此，时钟依赖性 PDF 信号传导每天塑造这些重要时钟神经元的连接。
We have previously reported that pigment dispersing factor (PDF) neurons, which are essential in the control of rest-activity cycles in Drosophila, undergo circadian remodeling of their axonal projections, a phenomenon called circadian structural plasticity, Axonal arborizations display higher complexity during the day and become simpler at night, and this remodeling involves changes in the degree of connectivity. This phenomenon depends on the clock present within the ventrolateral neurons (LNvs) as well as in glia. In this work, we characterize in detail the contribution of the PDF neuropeptide to structural plasticity at different times across the day. Using diverse genetic strategies to temporally restrict its downregulation, we demonstrate that even subtle alterations to PDF cycling at the dorsal protocerebrum correlate with impaired remodeling, underscoring its relevance for the characteristic morning spread; PDF released from the small LNvs (sLNvs) and the large LNvs (ILNvs) contribute to the process. Moreover, forced depolarization recruits activity-dependent mechanisms to mediate growth only at night, overcoming the restriction imposed by the clock on membrane excitability. Interestingly, the active process of terminal remodeling requires PDF receptor (PDFR) signaling acting locally through the cyclic-nucleotide-gated channel ion channel subunit A (CNGA). Thus, clock-dependent PDF signaling shapes the connectivity of these essential clock neurons on daily basis.