Versatile drug nanocarrier assembly via conjugation of distinct carbon dots
Versatile drug nanocarrier assembly via conjugation of distinct carbon dots
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DOI：
10.48317/imist.prsm/morjchem-v8i4.22304
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发表时间：
2020-08
影响因子：
1.1
通讯作者：
Yiqun Zhou;Jiuyan Chen;Nikolay Miloserdov;Wei Zhang;Keenan J. Mintz;B. Ferreira;M. Mićić;
中科院分区：
文献类型：
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作者：
Yiqun Zhou;Jiuyan Chen;Nikolay Miloserdov;Wei Zhang;Keenan J. Mintz;B. Ferreira;M. Mićić;
随着各种中枢神经系统相关疾病的不断发生和严重后果，药物传递在现代医学中的重要性逐渐显现。药物传递的最大挑战在于选择合适的药物传递载体。世纪是各种纳米材料蓬勃发展的世纪。由于纳米材料在纳米尺度上具有许多优异的性质，因此作为药物纳米载体得到了广泛的研究。作为碳基纳米材料家族的新成员，碳量子点（CD）已被证明是一种很有前途的药物纳米载体。它们已经成功地与各种治疗剂缀合用于靶向药物递送。然而，考虑到单一CD制剂在药物递送方面的局限性，在本研究中，两种不同的CD制剂（G-CD和Y-CD）被缀合以弥补彼此的不足。使用不同的透析袋来纯化CD缀合物（G-Y CD），并揭示小缀合物系统和大缀合物系统之间的差异。经过一系列的物理化学表征，G-Y CD表现出许多纳米载体有利的性质，如激发依赖的光致发光（PL），多样化的表面功能，可控的形态和通用的两亲性。为了进一步分析G-Y CD的形成机理，分别对G-CD和Y-CD进行了自共轭研究，结果表明Y-CD之间能够发生自共轭。最终，为了评估G-Y CD作为未来CNS相关疾病的药物纳米载体的能力，将G-Y CD血管内注射到斑马鱼的心脏中。脊髓中的荧光信号表明G-Y CD具有穿过血脑屏障（BBB）的能力。因此，这项研究揭示了一种新的策略，组装通用的药物纳米载体，通过共轭不同的CD。
With constant emergencies and severe consequences of various central nervous system (CNS)-related diseases, drug delivery gradually reveals its significance in the modern medicine. The biggest challenge of drug delivery resides in the selection of appropriate drug delivery carrier. 21 th century witnessed the prosperous development of diverse nanomaterials. Due to many excellent properties revealed in nanoscale, nanomaterials have been widely investigated as drug nanocarriers. As a new family member of carbon-based nanomaterials, carbon dots (CDs) have proved to be promising drug nanocarriers. They have been successfully conjugated with various therapeutic agents for targeted drug delivery. However, considering the limitation of single CD preparation in drug delivery, in this study, two distinct CD preparations (G-CDs and Y-CDs) were conjugated to compensate for each other’s deficiencies. Different dialysis bags were employed to purify the CD conjugate (G-Y CDs) and reveal the difference between small and large-conjugated systems. After a series of physicochemical characterizations, G-Y CDs exhibited many nanocarrier-favored properties such as excitation-dependent photoluminescence (PL), diversified surface functionality, controlled morphology and versatile amphiphilicity. To further analyze the formation mechanism of G-Y CDs, self-conjugation was separately surveyed with G-CDs and Y-CDs, which showed that self-conjugation was able to occur between Y-CDs. Eventually, to evaluate the capacity of G-Y CDs as drug nanocarriers for future CNS-related diseases, G-Y CDs were intravascularly injected into the heart of zebrafish. The fluorescence signal in the spinal cord suggested the capability of G-Y CDs to cross the blood-brain barrier (BBB). Therefore, this study reveals a novel strategy to assemble versatile drug nanocarriers through conjugation of distinct CDs.