Ginsenoside Rg1 attenuates dopamine-induced apoptosis in PC12 cells by suppressing oxidative stress
Ginsenoside Rg1 attenuates dopamine-induced apoptosis in PC12 cells by suppressing oxidative stress
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DOI：
10.1016/s0014-2999(03)01945-9
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发表时间：
2003-07-18
影响因子：
5
通讯作者：
Zhao, CH
中科院分区：
文献类型：
--
作者：
Chen, XC;Zhu, YG;Zhao, CH
在帕金森病中，已经提出了拯救多巴胺神经元的神经保护疗法。人参皂苷 Rg1 是人参的生物活性成分之一，可能是一种候选的神经保护药物。在本研究中，研究了人参皂苷 Rg1 对 PC12 细胞中外源多巴胺诱导的细胞凋亡提供神经保护的机制。人参皂苷 Rg1 预处理显着减少了多巴胺诱导的活性氧的产生以及线粒体细胞色素 c 向细胞质的释放，并随后抑制了 caspase-3 的激活。此外，Rg1 预处理还降低了诱导型一氧化氮 (NO) 合酶蛋白水平和 NO 产量。这些结果表明，人参皂苷 Rg1 可以通过抑制细胞内氧化应激来减弱多巴胺诱导的细胞凋亡，并且可以拯救或保护帕金森病中的多巴胺神经元。 (C) 2003 Elsevier B.V. 保留所有权利。
In Parkinson's disease, neuroprotective therapy to rescue dopamine neurons has been proposed. Ginsenoside Rg1, one of the biologically active ingredients of ginseng, may be a candidate neuroprotective drug. In the present study, the mechanism underlying the neuroprotection provided by ginsenosde Rg1 was studied against apoptosis induced by exogenous dopamine in PC12 cells. Pretreatment with ginsenoside Rg1 markedly reduced the generation of dopamine-induced reactive oxygen species and the release of mitochondrial cytochrome c into the cytosol, and subsequently inhibited the activation of caspase-3. In addition, Rg1 pretreatment also reduced inducible nitric oxide (NO) synthase protein level and NO production. These results suggested that ginsenoside Rg1 may attenuate dopamine-induced apoptotic cell death through suppression of intracellular oxidative stress, and that it, may rescue or protect doparnine neurons in Parkinson's disease. (C) 2003 Elsevier B.V. All rights reserved.