Effect of Soyabean Isoflavones Exposure on Onset of Puberty, Serum Hormone Concentration and Gene Expression in Hypothalamus, Pituitary Gland and Ovary of Female Bama Miniature Pigs.
Effect of Soyabean Isoflavones Exposure on Onset of Puberty, Serum Hormone Concentration and Gene Expression in Hypothalamus, Pituitary Gland and Ovary of Female Bama Miniature Pigs.
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DOI：
10.5713/ajas.15.0185
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Yin Y
中科院分区：
文献类型：
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作者：
Fan J;Zhang B;Li L;Xiao C;Oladele OA;Jiang G;Ding H;Wang S;Xing Y;Xiao D;Yin Y
本试验旨在研究大豆异黄酮（SIF）对雌性巴马小型猪青春期开始、血清激素浓度以及下丘脑、垂体和卵巢基因表达的影响。选取55头35日龄猪，随机分为5个处理组，每个处理11头猪。结果表明，在基础饲粮中添加不同剂量（0、250、500和1250 mg/kg）的SIF可显著提高饲粮中添加1250 mg/kg SIF的猪的青春期延迟（p<0.05）。添加SIF或戊酸雌二醇（EV）降低了4月龄猪血清促性腺激素释放激素和黄体生成素浓度（p<0.05），但提高了促卵泡激素浓度。在sif添加组中，KISS1转移抑制因子（KISS1）、甾体急性调节蛋白（StAR）和3- β-羟基类固醇脱氢酶/ δ -5- δ -4异构酶（3β-HSD）的表达均降低（p<0.01）。暴露于250、1,250 mg/kg SIF和EV时，小型猪垂体促性腺激素释放激素受体的表达量较对照组降低（p<0.05）。与对照组相比，250 mg/kg SIF和EV组细胞色素P450 19A1的表达也显著降低（p<0.05）。我们的研究结果表明，膳食补充SIF会导致青春期延迟，这可能是由于在类固醇激素合成中起重要作用的关键基因下调所致。
This study was to investigate the effect of soyabean isoflavones (SIF) on onset of puberty, serum hormone concentration, and gene expression in hypothalamus, pituitary and ovary of female Bama miniature pigs. Fifty five, 35-days old pigs were randomly assigned into 5 treatment groups consisting of 11 pigs per treatment. Results showed that dietary supplementation of varying dosage (0, 250, 500, and 1,250 mg/kg) of SIF induced puberty delay of the pigs with the age of puberty of pigs fed basal diet supplemented with 1,250 mg/kg SIF was significantly higher (p<0.05) compared to control. Supplementation of SIF or estradiol valerate (EV) reduced (p<0.05) serum gonadotrophin releasing hormone and luteinizing hormone concentration, but increased follicle-stimulating hormone concentration in pigs at 4 months of age. The expression of KiSS-1 metastasis-suppressor (KISS1), steroidogenic acute regulatory protein (StAR) and 3-beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase (3β-HSD) was reduced (p<0.01) in SIF-supplemented groups. Expression of gonadotropin-releasing hormone receptor in the pituitary of miniature pigs was reduced (p<0.05) compared to the control when exposed to 250, 1,250 mg/kg SIF and EV. Pigs on 250 mg/kg SIF and EV also showed reduced (p<0.05) expression of cytochrome P450 19A1 compared to the control. Our results indicated that dietary supplementation of SIF induced puberty delay, which may be due to down-regulation of key genes that play vital roles in the synthesis of steroid hormones.