Optical studies of early developing cardiac and neural activities using voltage-sensitive dyes.
Optical studies of early developing cardiac and neural activities using voltage-sensitive dyes.
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使用电压敏感染料对早期发育的心脏和神经活动进行光学研究。
DOI：
10.2170/jjphysiol.40.443
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Kamino
中科院分区：
文献类型：
--
作者：
K. Kamino
使用基于电压敏感染料的监测细胞电活动的光学方法，我们已经克服了在胚胎心脏和中枢神经系统发育早期的电功能研究的几个障碍。我们已经能够监测，第一次，自发电活动的预融合的心脏原基在早期鸡胚在6-和早期7体节发育阶段，并遵循早期发展的电活动在收缩前的心脏在7- 9体节阶段。此外，我们还用光学方法监测了早期鸡胚脑干的神经反应，并确定了反应的空间模式。
Using optical methods for monitoring cellular electrical activity based on voltage-sensitive dyes, we have overcome several obstacles to the study of electrical function in the embryonic heart and central nervous system during early development. We have been able to monitor, for the first time, spontaneous electrical activity in the pre-fused cardiac primordia in early chick embryos at the 6- and early 7-somite stages of development and to follow the early development of electrical activity in the pre-contractile heart at the 7- to 9-somite stages. In addition, we have monitored neural responses in the early embryonic chick brain stem by optical means, and determined the spatial pattern of the response.