NRT1.1B is associated with root microbiota composition and nitrogen use in field-grown rice
NRT1.1B is associated with root microbiota composition and nitrogen use in field-grown rice
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NRT1.1B 与田间种植水稻的根部微生物群组成和氮利用相关
DOI：
10.1038/s41587-019-0104-4
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发表时间：
2019-06-01
影响因子：
46.9
通讯作者：
Bai, Yang
中科院分区：
文献类型：
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作者：
Zhang, Jingying;Liu, Yong-Xin;Bai, Yang
籼稻品种氮素利用效率上级优于粳稻品种。我们应用16S核糖体RNA基因分析来表征68个籼稻和27个粳稻品种在田间生长的根微生物群。我们发现，籼稻和粳稻招募不同的根微生物群。值得注意的是，富含籼稻的细菌类群更多样化，包含更多的属与氮代谢功能，比富含粳稻的类群。利用遗传学方法，我们提供的证据表明，NRT1.1B，水稻硝酸盐转运蛋白和传感器，是与招聘的一个大比例的富含籼稻的细菌。宏基因组测序显示，氨化过程在nrt1.1b突变体的根微生物组中不太丰富。我们从籼稻和粳稻根中分离出1，079个纯细菌分离物并衍生出合成群落（SynComs）。与富含粳稻的SynCom相比，在有机氮条件下，接种富含籼稻的SynCom的籼稻品种IR24提高了水稻的生长。本研究中建立的植物基因型和根微生物群成员之间的联系将为育种策略提供信息，以提高作物的氮素利用率。水稻协调根系微生物群的招募，以优化从土壤中获取氮素。
Nitrogen-use efficiency of indica varieties of rice is superior to that of japonica varieties. We apply 16S ribosomal RNA gene profiling to characterize root microbiota of 68 indica and 27 japonica varieties grown in the field. We find that indica and japonica recruit distinct root microbiota. Notably, indica-enriched bacterial taxa are more diverse, and contain more genera with nitrogen metabolism functions, than japonica-enriched taxa. Using genetic approaches, we provide evidence that NRT1.1B, a rice nitrate transporter and sensor, is associated with the recruitment of a large proportion of indica-enriched bacteria. Metagenomic sequencing reveals that the ammonification process is less abundant in the root microbiome of the nrt1.1b mutant. We isolated 1,079 pure bacterial isolates from indica and japonica roots and derived synthetic communities (SynComs). Inoculation of IR24, an indica variety, with an indica-enriched SynCom improved rice growth in organic nitrogen conditions compared with a japonica-enriched SynCom. The links between plant genotype and root microbiota membership established in this study will inform breeding strategies to improve nitrogen use in crops. Rice coordinates recruitment of the root microbiota to optimize nitrogen acquisition from soil.