Label-Free Detection of Clustering of Membrane-Bound Proteins
Label-Free Detection of Clustering of Membrane-Bound Proteins
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DOI：
10.1021/ac102495q
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发表时间：
2010-11-15
影响因子：
7.4
通讯作者：
Richter, Ralf P.
中科院分区：
文献类型：
--
作者：
Carton, Ixaskun;Brisson, Alain R.;Richter, Ralf P.
我们报告了一种新的无标记方法来检测和表征膜结合蛋白的聚集，并通过扩展来检测和表征液体环境中吸附到平面表面的纳米颗粒的横向分离。该方法利用两种不同的质量和表面敏感检测方法（具有耗散监测的石英晶体微天平和椭圆光度法）之间的对比。两种技术的时间分辨相关性提供了对表面结合分子聚类状态的微妙变化的洞察，这是单独使用任何一种技术都无法实现的。理论模型可以提供有关表面束缚簇尺寸的定量预测。
We report a new and label-free method to detect and characterize the clustering of membrane-bound proteins and, by extension, the lateral segregation of nanosized particles adsorbed to planar surfaces in liquid environment. The method exploits the contrast between two different mass and surface sensitive detection methods, quartz crystal microbalance with dissipation monitoring and ellipsometry. The time-resolved correlation of both techniques provides insight into subtle changes in the clustering state of surface-bound molecules that is not accessible with either technique alone. A theoretical model can provide quantitative predictions about the size of surface-bound clusters.