Improved production of galanthamine and related alkaloids by methyl jasmonate in Narcissus confusus shoot-clumps
Improved production of galanthamine and related alkaloids by methyl jasmonate in Narcissus confusus shoot-clumps
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DOI：
10.1055/s-2004-835849
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发表时间：
2004-12-01
期刊：
影响因子：
2.7
通讯作者：
Codina, C
中科院分区：
文献类型：
--
作者：
Colque, R;Viladomat, F;Codina, C
用茉莉酸甲酯、花生四烯酸、壳聚糖和水杨酸等生物诱导剂处理南方水仙的液体振荡培养茎丛。研究了这些化合物对外植体生长的影响，以及在实验结束时释放到液体培养基中并在组织中积累的生物碱的量。所获得的结果表明，一般而言，高剂量的这些化合物对外植体的生长具有负面影响，特别是水杨酸。相反，茉莉酸甲酯的加入，主要是在25 μ M，促进加兰他敏和其他相关生物碱的释放到液体介质中的比例高达300%，相对于对照外植体，也在组织中积累。研究的其他激发子对这些石蒜科类型生物碱的产生没有任何有趣的影响。
Liquid-shake cultured shoot-clumps of Narcisus confussus were treated with the commonly used biotic elicitors methyl jasmonate, arachidonic acid, chitosan and salicylic acid. The effects of these compounds on the growth of the explants, as well as on the amount of the alkaloids released to the liquid culture medium and accumulated in the tissues at the end of the experiment were studied. The obtained results showed that, in general, high doses of these compounds had a negative effect on the growth of the explants, particularly the salicylic acid. On the contrary, the addition of methyljasmonate, mainly at 25 muM, promoted the release of galanthamine and other related alkaloids to the liquid medium in proportions of up to 300% in relation to the control explants, and also their accumulation in tissues. The other elicitors studied did not have any interesting effects on the production of these Amaryllidaceae-type alkaloids.