Functionalized graphene as sensitive electrochemical label in target-dependent linkage of split aptasensor for dual detection
Functionalized graphene as sensitive electrochemical label in target-dependent linkage of split aptasensor for dual detection
复制标题
功能化石墨烯作为敏感电化学标记，用于分裂适体传感器的目标依赖性连接，用于双重检测
DOI：
10.1016/j.bios.2014.06.008
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发表时间：
2014-12-15
影响因子：
12.6
通讯作者：
Qu, Xiaogang
中科院分区：
文献类型：
--
作者：
Feng, Lingyan;Zhang, Zhijun;Qu, Xiaogang
本文报道了一种新型的电化学适体传感器，以功能化的石墨烯作为高效的电化学标记物，实现了对腺苷三磷酸（ATP）和腺苷脱氨酶（ADA）活性的高灵敏检测。ATP与其适配体的特异性结合可以将裂解的适配体修饰的石墨烯与磁珠连接在一起。在ADA催化和磁分离后，石墨烯材料锚定在电极表面将有效地促进电子转移，从而产生可检测的电化学信号。ATP和ADA活性的检测限分别为13.6 nM和0.01单位/mL（与1.2 nM相似）。我们的工作将为石墨烯基材料在生物医学和生物传感器中的诊断应用提供新的视野。(C)2014爱思唯尔有限公司版权所有。
A new type of electrochemical aptasensor was reported here for highly sensitive detection of adenosine triphosphate (ATP) and adenosine deaminase (ADA) activity using functionalized graphene as efficient electrochemical label. The specific binding of ATP and its aptamer could link the split aptamers modified graphene and magnetic beads together. After ADA catalysis and magnetic separation, graphene material anchored on electrode surface would efficiently facilitate electron transfer, thus produce detectable electrochemical signals. The detection limits for ATP and ADA activity were 13.6 nM and 0.01 unit/mL (similar to 1.2 nM), respectively. Our work would supply new horizons for the diagnostic applications of graphene-based materials in biomedicine and biosensors. (C) 2014 Elsevier B.V. All rights reserved.