Chemotaxis towards pteridines during development of Dictyostelium.
Chemotaxis towards pteridines during development of Dictyostelium.
复制标题
盘基网柄菌发育过程中对蝶啶的趋化性。
作者：
H. S. Tillinghast;P. Newell
在先前的研究表明，在盘基网柄藻的发育阶段，叶酸的敏感性，一系列的蝶啶类药物进行了研究，他们的能力，以诱导阿米巴趋化性在发展过程中，这种生物体。几种化合物被发现类似于叶酸在其诱导营养阿米巴和阿米巴在饥饿的最初几个小时内发展的趋化性的能力。一种化合物，L-单喋呤，是独特的，在显示趋化活性，只有在发育阶段后，约12小时的饥饿。使用细胞骨架肌动蛋白的聚合作为与趋化性相关的细胞反应的测定的测试表明，10 nM-L-monapterin是这种反应的有效诱导剂，并且仅在12小时的发展后才出现反应。这些事件的时间可能与形成的提示含有柄细胞发生在多细胞聚集体在约12小时，并建议的作用，L-单喋呤（或天然存在的，密切相关的蝶啶）在细胞分选。讨论了蝶啶类化合物的进化意义。
Following a previous study indicating a sensitivity to folate during the developmental phase of Dictyostelium discoideum, a series of pteridines were investigated for their ability to induce amoebal chemotaxis during development of this organism. Several compounds were found to resemble folate in their ability to induce chemotaxis of both vegetative amoebae and amoebae developing during the first few hours of starvation. One compound, L-monapterin, was distinct in showing chemotactic activity only during the developmental phase after approximately 12 h of starvation. Tests using the polymerization of cytoskeletal actin as an assay for a cellular response correlated with chemotaxis showed that 10 nM-L-monapterin was a potent inducer of this response and that responsiveness appeared only after 12 h of development. The timing of these events may be correlated with the formation of tips containing stalk cells that occurs in multicellular aggregates at approximately 12 h, and suggests a role for L-monapterin (or a naturally occurring, closely related pteridine) in cell sorting. The evolutionary significance of the roles of pteridines is discussed.