Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans.
Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans.
复制标题
线虫秀丽隐杆线虫 I 号染色体精子发生缺陷突变体的发育遗传学。
DOI：
10.1093/genetics/120.2.435
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Ward,S
中科院分区：
文献类型：
--
作者：
L'Hernault,SW;Shakes,DC;Ward,S
影响秀丽隐杆线虫精子发生的突变可以用来解剖减数分裂和精子形态成熟的过程。我们获得了23个新的影响精子发生的染色体I突变（spe突变）。这些突变与先前描述的6个突变一起鉴定出11个互补群，其中6个由多个等位基因定义。这些spe突变都是隐性的，导致正常的自育雌雄同体产生未受精的卵母细胞，这些卵母细胞可以被野生型男性精子受精。5个1号染色体突变/缺陷杂合子具有与纯合子相似的表型，表明这些基因的零表型可能是有缺陷的精子。精子发生在不同的步骤被这些基因的突变所破坏。1度精母细胞的成熟被spe-4和spe-5的突变所破坏。来自sp8和sp12突变体的精子在雄性中发育为正常精子，而在雌雄同体中则不能。铁-6精子是异常的，铁-1精子看起来正常，但随后变成异常精子。5个基因（fe -7、spe-9、spe-11、spe-13和spe-15）发生突变，可以形成看起来正常的可运动精子，而这些精子在精子-精囊细胞或精子-卵母细胞的相互作用中似乎存在缺陷。
Mutations affecting Caenorhabditis elegans spermatogenesis can be used to dissect the processes of meiosis and spermatozoan morphological maturation. We have obtained 23 new chromosome I mutations that affect spermatogenesis (spe mutations). These mutations, together with six previously described mutations, identify 11 complementation groups, of which six are defined by multiple alleles. These spe mutations are all recessive and cause normally self-fertile hermaphrodites to produce unfertilized oocytes that can be fertilized by wild-type male sperm. Five chromosome I mutation/deficiency heterozygotes have similar phenotypes to the homozygote showing that the probable null phenotype of these genes is defective sperm. Spermatogenesis is disrupted at different steps by mutations in these genes. The maturation of 1 degree spermatocytes is disrupted by mutations in spe-4 and spe-5. Spermatids from spe-8 and spe-12 mutants develop into normal spermatozoa in males, but not in hermaphrodites. fer-6 spermatids are abnormal, and fer-1 spermatids look normal but subsequently become abnormal spermatozoa. Mutations in five genes (fer-7, spe-9, spe-11, spe-13 and spe-15) allow formation of normal looking motile spermatozoa that appear to be defective in either sperm-spermathecal or sperm-oocyte interactions.