A new pipeline for clinico-pathological and molecular placental research utilizing FFPE tissues
A new pipeline for clinico-pathological and molecular placental research utilizing FFPE tissues
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DOI：
10.1016/j.placenta.2021.07.301
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发表时间：
2021-09-01
期刊：
影响因子：
3.8
通讯作者：
Penn, Anna A.
中科院分区：
文献类型：
--
作者：
O'Reilly, Jiaqi J.;Barak, Stephanie;Penn, Anna A.
胎盘是许多妊娠病理学的核心，但由于两个关键的研究障碍，我们对胎盘功能的了解有限：1）缺乏样本收集和病理诊断的指南; 2）有限的工具可用于对储存的胎盘样本进行分子分析。我们的目的是建立一个可搜索的，基于人群的病理诊断胎盘数据库，并验证匹配福尔马林固定石蜡包埋（FFPE）胎盘块的基因表达研究的分子方法。我们的数据库中有超过1000例妊娠编码用于临床诊断，相应的FFPE块可用于基因表达研究。从FFPE组织收获的RNA具有足够的质量用于下游应用。我们成功地使用该管道从足月妊娠和早产妊娠中鉴定了FFPE胎盘，并比较了它们的基因表达。该平台的建立，将临床病理数据和分子基因表达联系起来，将增加我们对产科疾病的理解。
The placenta is at the core of many pregnancy pathologies, but we have limited knowledge about placental function because of two key research barriers: 1) lack of guidelines for sample collection and pathologic diagnosis; and 2) limited tools are available for molecular analysis of stored placental samples. We aimed to create a searchable, population-based placental database of pathologic diagnoses, and to validate molecular methods for gene expression studies of matching formalin fixed paraffin embedded (FFPE) placental blocks. Our database has over 1000 pregnancies coded for clinical diagnosis with corresponding FFPE blocks that are available for gene expression studies. RNA harvested from FFPE tissues is of sufficient quality for downstream applications. We successfully used this pipeline to identify FFPE placenta from term and preterm pregnancies, and compared their gene expression. The establishment of this platform, which links clinicopathological data and molecular gene expression, will increase our understanding of obstetrical diseases.