Plasma GLP-2 levels and intestinal markers in the juvenile pig during intestinal adaptation: Effects of different diet regimens
Plasma GLP-2 levels and intestinal markers in the juvenile pig during intestinal adaptation: Effects of different diet regimens
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DOI：
10.1023/b:ddas.0000043388.52260.2f
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Binesm, JE
中科院分区：
文献类型：
--
作者：
Paris, MC;Fuller, PJ;Binesm, JE
切除后残余小肠的适应取决于腔内和体液因素。我们的目的是确定胰高血糖素样肽（GLP-2）的循环水平是否在切除后的不同饮食方案下发生变化，并确定血浆GLP-2水平与肠道适应标志物之间是否存在关系。4周龄仔猪接受75%近端小肠切除（n = 31）或横切（n = 14）。术后接受猪饲料（n = 14），非聚合（元素）婴儿配方奶粉（n = 7），或单独使用聚合婴儿配方奶粉（n = 8），或补充纤维（n = 6）或牛初乳浓缩蛋白（CPC, n = 10），持续8周直至祭祀。分别于术后第0、2、4、8周测定血浆GLP-2水平。此外，研究了第8周的末期参数，包括体重增加、回肠绒毛高度、隐窝深度和双糖酶水平。切除动物的血浆GLP-2水平高于喂食相同饮食的横切动物。与其他饲粮组相比，初乳分离蛋白补充动物切除后血浆GLP-2水平显著升高。血浆GLP-2 （pM）的升高在术后前两周最大（第0周，15.5；第2周，30.9），随后在第2至4周达到平稳期，第4至8周GLP-2水平下降。在第8周，血浆GLP-2水平与体重增加、绒毛高度、乳糖酶、蔗糖酶、麦芽糖酶、隐窝深度或绒毛/隐窝比之间没有关系。血浆GLP-2水平在大量小肠切除术后的第一周升高。饲粮中添加CPC可提高血浆GLP-2水平。本研究中观察到的GLP-2水平的变化可能表明，GLP-2在该临床前模型中切除后肠道的适应性反应中发挥作用。
Adaptation of the residual small bowel following resection is dependent on luminal and humoral factors. We aimed to establish if circulating levels of glucagon-like peptide (GLP-2) change under different dietary regimens following resection and to determine if there is a relationship between plasma GLP-2 levels and markers of intestinal adaptation. Four-week-old piglets underwent a 75% proximal small bowel resection (n = 31) or transection (n = 14). Postoperatively they received either pig chow (n = 14), nonpolymeric (elemental) infant formula (n = 7), or polymeric infant formula alone (n = 8) or supplemented either with fiber (n = 6) or with bovine colostrum protein concentrate (CPC; n = 10) for 8 weeks until sacrifice. Plasma GLP-2 levels were measured at weeks 0, 2, 4, and 8 postoperatively. In addition, end-stage parameters were studied at week 8 including weight gain, ileal villus height, crypt depth, and disaccharidase levels. Plasma GLP-2 levels were higher in resected animals compared to transected animals fed the same diet. Plasma GLP-2 levels were significantly increased in the colostrum protein isolate-supplemented animals following resection compared to all other diet groups. The increase in plasma GLP-2 (pM) was greatest in the first 2 weeks postresection (week 0, 15.5; week 2, 30.9), followed by a plateau at weeks 2 to 4 and a decrease in GLP-2 levels from week 4 to week 8. At week 8, no relationships were found between the plasma GLP-2 levels and the measurements of weight gain, villus height, lactase, sucrase, maltase, crypt depth, or villus/crypt ratio. Plasma GLP-2 levels increase in the first weeks following massive small intestinal resection. The increase in plasma GLP-2 levels was enhanced by supplementation of the diet with CPC. The changes in GLP-2 levels observed in this study may suggest that GLP-2 plays a role in the adaptive response in the intestine following resection in this preclinical model.