Support for a meiotic recombination initiation complex: Interactions among Rec102p, Rec104p, and Spo11p
Support for a meiotic recombination initiation complex: Interactions among Rec102p, Rec104p, and Spo11p
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DOI：
10.1128/mcb.23.16.5928-5938.2003
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发表时间：
2003-08-01
影响因子：
5.3
通讯作者：
Malone, R
中科院分区：
文献类型：
--
作者：
Jiao, K;Salem, L;Malone, R
在酿酒酵母中启动减数分裂重组需要至少10个基因产物。起始事件产生双链断裂，然后由其他重组酶处理。各种经典的观察，如重组结节的存在，表明催化重组的蛋白质形成复合物。各种证据表明Rad50p、Mre11p和Xrs2p相互作用，并且已经报道了表明Rec102p和Rec104p之间相互作用的遗传数据。最近的研究表明，Spo11p与Rec102p在减数分裂过程中也发生免疫共沉淀。在本文中，我们提供的遗传和生化证据表明，减数分裂特异性蛋白Rec102p，Rec104p和Spo11p都在减数分裂中相互作用。此外，我们证明了Rec102p和Spo11p之间的相互作用不需要Rec104p。同样，Rec104p和Rec102p之间的相互作用不需要Spo11p，尽管Spo11p可以稳定这种关联。的相互作用表明，Spo11p，Rec102p和Rec104p可能会形成一个三聚体复合物在重组的启动。
Initiation of meiotic recombination in the yeast Saccharomyces cerevisiae requires at least 10 gene products. The initiation event creates double-strand breaks, which are then processed by other recombination enzymes. A variety of classical observations, such as the existence of recombination nodules, have suggested that the proteins catalyzing recombination form a complex. A variety of lines of evidence indicate that Rad50p, Mre11p, and Xrs2p interact, and genetic data suggesting interactions between Rec102p and Rec104p have been reported. It has recently been shown that Spo11p coimmunoprecipitates with Rec102p in meiosis as well. In this paper, we provide genetic and biochemical evidence that the meiosis-specific proteins Rec102p, Rec104p, and Spo11p all interact with each other in meiosis. Furthermore, we demonstrate that the interaction between Rec102p and Spo11p does not require Rec104p. Likewise, the interaction between Rec104p and Rec102p does not require Spo11p, although Spo11p may stabilize that association. The interactions suggest that Spo11p, Rec102p, and Rec104p may form a trimeric complex during the initiation of recombination.